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Literature summary for 6.3.2.1 extracted from

  • Miyatake, K.; Nakano, Y.; Kitaoka, S.
    Enzymological properties of pantothenate synthetase from Escherichia coli B (1978), J. Nutr. Sci. Vitaminol., 24, 243-253.
    View publication on PubMed

General Stability

General Stability Organism
lyophilized enzyme is very stable Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Escherichia coli
2,3-Diaminopropanoate slight Escherichia coli
2-hydroxybutanoate slight Escherichia coli
2-Hydroxypropanoate slight Escherichia coli
3-hydroxybutanoate slight Escherichia coli
3-hydroxypropanoate slight Escherichia coli
4-Amino-3-hydroxybutanoate
-
Escherichia coli
4-aminobutanoate
-
Escherichia coli
5-Aminopentanoate slight Escherichia coli
6-aminohexanoate slight Escherichia coli
Ag+
-
Escherichia coli
anserine slight Escherichia coli
Ba2+
-
Escherichia coli
Ca2+
-
Escherichia coli
carnosine slight Escherichia coli
Co2+
-
Escherichia coli
Cu2+
-
Escherichia coli
EDTA
-
Escherichia coli
EGTA
-
Escherichia coli
Fe2+
-
Escherichia coli
gluconate 32% inhibition at 1 mM Escherichia coli
glycolate 30% inhibition at 1 mM Escherichia coli
Hg2+
-
Escherichia coli
iodoacetate
-
Escherichia coli
L-Ala
-
Escherichia coli
L-cysteate
-
Escherichia coli
L-Val
-
Escherichia coli
Lactate
-
Escherichia coli
Ni2+ slight Escherichia coli
Sn2+ slight Escherichia coli
Sr2+ slight Escherichia coli
taurine
-
Escherichia coli
Zn2+
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
D-pantoate
-
Escherichia coli
0.1
-
ATP
-
Escherichia coli
0.15
-
beta-Alanine
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ combination of divalent and monovalent cation required Escherichia coli
Mg2+ divalent cation required, Mg2+ or Mn2+, Km: 2.0 mM. Optimal concentration: 10 mM, inhibition above Escherichia coli
Mn2+ combination of divalent and monovalent cation required Escherichia coli
Mn2+ divalent cation required, Mg2+ or Mn2+. Optimal concentration: 5 mM, inhibition above Escherichia coli
NH4+ combination of divalent and monovalent cation required Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate bi uni uni bi ping pong mechanism Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, stable for 6 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantoate + beta-alanine
-
Escherichia coli AMP + diphosphate + (R)-pantothenate
-
?
CTP + (R)-pantoate + beta-alanine 14% of the activity relative to ATP Escherichia coli CMP + diphosphate + (R)-pantothenate
-
?
GTP + (R)-pantoate + beta-alanine 12% of the activity relative to ATP Escherichia coli GMP + diphosphate + (R)-pantothenate
-
?
ITP + (R)-pantoate + beta-alanine 14% of the activity relative to ATP Escherichia coli IMP + diphosphate + (R)-pantothenate
-
?
UTP + (R)-pantoate + beta-alanine 16% of the activity relative to ATP Escherichia coli UMP + diphosphate + (R)-pantothenate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
10 min, complete loss of activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 15 min, 40°C, stable Escherichia coli