General Stability | Organism |
---|---|
lyophilized enzyme is very stable | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Escherichia coli | |
4-Amino-3-hydroxybutanoate | 32% inhibition at 1 mM | Escherichia coli | |
4-aminobutanoate | 27% inhibition at 1 mM | Escherichia coli | |
Ag+ | - |
Escherichia coli | |
aspartate | 24% inhibition at 1 mM | Escherichia coli | |
Ba2+ | - |
Escherichia coli | |
Ca2+ | - |
Escherichia coli | |
Co2+ | - |
Escherichia coli | |
Cu2+ | - |
Escherichia coli | |
EDTA | - |
Escherichia coli | |
EGTA | - |
Escherichia coli | |
Fe2+ | slight | Escherichia coli | |
gluconate | 32% inhibition at 1 mM | Escherichia coli | |
glycolate | 30% inhibition at 1 mM | Escherichia coli | |
Hg2+ | - |
Escherichia coli | |
methylmalonate | 38% inhibition at 1 mM | Escherichia coli | |
Mg2+ | inhibition above 10 mM | Escherichia coli | |
Mn2+ | inhibition above 5 mM | Escherichia coli | |
Ni2+ | slight | Escherichia coli | |
Pb2+ | slight | Escherichia coli | |
Sn2+ | slight | Escherichia coli | |
Sr2+ | slight | Escherichia coli | |
taurine | 52% inhibition at 1 mM | Escherichia coli | |
Zn2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
D-pantoate | - |
Escherichia coli | |
0.1 | - |
ATP | - |
Escherichia coli | |
0.15 | - |
beta-Alanine | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates | Escherichia coli | |
K+ | Km: 5.5 mM | Escherichia coli | |
Mg2+ | divalent cation required, Mg2+ or Mn2+, Km: 2.0 mM. Optimal concentration: 10 mM, inhibition above | Escherichia coli | |
Mn2+ | divalent cation required, Mg2+ or Mn2+. Optimal concentration: 5 mM, inhibition above | Escherichia coli | |
NH4+ | activates | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
x * 18000, SDS-PAGE in presence of 2-mercaptoethanol | Escherichia coli |
69000 | - |
gel filtration | Escherichia coli |
70000 | - |
sedimentation equilibrium ultracentrifugation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
IFO 13168 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.05 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, stable for 6 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | - |
Escherichia coli | AMP + diphosphate + (R)-pantothenate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 18000, SDS-PAGE in presence of 2-mercaptoethanol | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
pH 5.0-10.0, 15 min stable | Escherichia coli |