Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.1 extracted from

  • Kumar, A.; Casey, A.; Odingo, J.; Kesicki, E.A.; Abrahams, G.; Vieth, M.; Masquelin, T.; Mizrahi, V.; Hipskind, P.A.; Sherman, D.R.; Parish, T.
    A high-throughput screen against pantothenate synthetase (PanC) identifies 3-biphenyl-4-cyanopyrrole-2-carboxylic acids as a new class of inhibitor with activity against Mycobacterium tuberculosis (2013), PLoS ONE, 8, e72786.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene panC, expression of His6-tagged maltose-binding protein fusion enzyme in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
3-(biphenyl-4-yl)-4-cyano-5-(ethylsulfanyl)-1-methyl-1H-pyrrole-2-carboxylic acid
-
Mycobacterium tuberculosis
4-cyano-3-(4'-cyanobiphenyl-4-yl)-1-methyl-5-propyl-1H-pyrrole-2-carboxylic acid
-
Mycobacterium tuberculosis
additional information evaluation of 3-biphenyl-4-cyanopyrrole-2-carboxylic acids as enzyme inhibitors, overview Mycobacterium tuberculosis
nafronyl oxalate
-
Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-pantoate + beta-alanine Mycobacterium tuberculosis
-
AMP + diphosphate + (R)-pantothenate
-
?
ATP + (R)-pantoate + beta-alanine Mycobacterium tuberculosis H37Rv
-
AMP + diphosphate + (R)-pantothenate
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WIL5 gene panC, locus Rv3602c
-
Mycobacterium tuberculosis H37Rv P9WIL5 gene panC, locus Rv3602c
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged maltose-binding protein fusion enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantoate + beta-alanine
-
Mycobacterium tuberculosis AMP + diphosphate + (R)-pantothenate
-
?
ATP + (R)-pantoate + beta-alanine
-
Mycobacterium tuberculosis H37Rv AMP + diphosphate + (R)-pantothenate
-
?

Synonyms

Synonyms Comment Organism
PanC
-
Mycobacterium tuberculosis
Pantothenate synthetase
-
Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000174
-
3-(biphenyl-4-yl)-4-cyano-5-(ethylsulfanyl)-1-methyl-1H-pyrrole-2-carboxylic acid versus pantoate, pH 7.8, 37°C Mycobacterium tuberculosis
0.000297
-
4-cyano-3-(4'-cyanobiphenyl-4-yl)-1-methyl-5-propyl-1H-pyrrole-2-carboxylic acid versus pantoate, pH 7.8, 37°C Mycobacterium tuberculosis