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Literature summary for 6.3.2.1 extracted from

  • Satoh, A.; Konishi, S.; Tamura, H.; Stickland, H.G.; Whitney, H.M.; Smith, A.G.; Matsumura, H.; Inoue, T.
    Substrate-induced closing of the active site revealed by the crystal structure of pantothenate synthetase from Staphylococcus aureus (2010), Biochemistry, 49, 6400-6410.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of the apoenzyme and the reaction intermediate complex are determined by X-ray crystallography to resolutions of 2.5 A and 1.85 A, respectively. Structural analysis indicate that the apoenzyme adopts an open and relatively mobile structure, while the complex structure is closed and entirely rigid. In the complex structure, pantothenate synthetase and acetate are bound in the active site. Acetate might mimic the substrate beta-alanine. Therefore, the complex structure might represent a catalytic state poised for in-line nucleophilic attack on pantothenate synthetase Staphylococcus aureus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus Q2FV22
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantoate + beta-alanine
-
Staphylococcus aureus AMP + diphosphate + (R)-pantothenate
-
?

Synonyms

Synonyms Comment Organism
Pantothenate synthetase
-
Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
beta-Alanine co-substrate: (R)-pantoate, pH 8.0, 30°C Staphylococcus aureus
1.5
-
(R)-pantoate co-substrate: beta-alanine, pH 8.0, 30°C Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Staphylococcus aureus

pH Range

pH Minimum pH Maximum Comment Organism
8 9
-
Staphylococcus aureus