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Literature summary for 6.3.2.1 extracted from

  • Jonczyk, R.; Ronconi, S.; Rychlik, M.; Genschel, U.
    Pantothenate synthetase is essential but not limiting for pantothenate biosynthesis in Arabidopsis (2008), Plant Mol. Biol., 66, 1-14.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene panC, recombinant expression as His-tagged enzyme, and expression in and complementation of the Arabidopsis thaliana knockout mutant phenotype by heterologous expression of Escherichia coli PTS, the panC transgene increases the total PTS activity in leaves of trangenic plants by up to 500fold but does not affect the steady-state level of pantothenate, overview Escherichia coli
promoter:beta-glucuronidase analysis Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of an uxotrophic AtPTS knockout mutant, phenotype, overview. Complementation of the mutant phenotype by heterologous expression of Escherichia coli PTS gene panC. The panC transgene increases the total PTS activity in leaves by up to 500fold but does not affect the steady-state level of pantothenate. The AtPTS knockout phenotype is rescued by exogenous pantothenate Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli
Mg2+
-
Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-pantoate + beta-alanine Escherichia coli
-
AMP + diphosphate + (R)-pantothenate
-
?
ATP + (R)-pantoate + beta-alanine Arabidopsis thaliana
-
AMP + diphosphate + (R)-pantothenate
-
?
additional information Arabidopsis thaliana pantothenate synthetase is essential but not limiting for pantothenate biosynthesis in Arabidopsis thaliana, pantothenate, i.e. vitamin B5, is the universal precursor for coenzyme A, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FKB3 ecotype Columbia-0, single gene
-
Escherichia coli
-
gene panC
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-
seed transcript and metabolite patterns of CoA biosynthesis during seed development involving the enzyme, overview Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantoate + beta-alanine
-
Escherichia coli AMP + diphosphate + (R)-pantothenate
-
?
ATP + (R)-pantoate + beta-alanine
-
Arabidopsis thaliana AMP + diphosphate + (R)-pantothenate
-
?
additional information pantothenate synthetase is essential but not limiting for pantothenate biosynthesis in Arabidopsis thaliana, pantothenate, i.e. vitamin B5, is the universal precursor for coenzyme A, overview Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
Pantothenate synthetase
-
Escherichia coli
Pantothenate synthetase
-
Arabidopsis thaliana
PTS
-
Escherichia coli
PTS
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Escherichia coli
9
-
assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli
ATP
-
Arabidopsis thaliana