Cloned (Comment) | Organism |
---|---|
gene panC, recombinant expression as His-tagged enzyme, and expression in and complementation of the Arabidopsis thaliana knockout mutant phenotype by heterologous expression of Escherichia coli PTS, the panC transgene increases the total PTS activity in leaves of trangenic plants by up to 500fold but does not affect the steady-state level of pantothenate, overview | Escherichia coli |
promoter:beta-glucuronidase analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an uxotrophic AtPTS knockout mutant, phenotype, overview. Complementation of the mutant phenotype by heterologous expression of Escherichia coli PTS gene panC. The panC transgene increases the total PTS activity in leaves by up to 500fold but does not affect the steady-state level of pantothenate. The AtPTS knockout phenotype is rescued by exogenous pantothenate | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli | |
Mg2+ | - |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | Escherichia coli | - |
AMP + diphosphate + (R)-pantothenate | - |
? | |
ATP + (R)-pantoate + beta-alanine | Arabidopsis thaliana | - |
AMP + diphosphate + (R)-pantothenate | - |
? | |
additional information | Arabidopsis thaliana | pantothenate synthetase is essential but not limiting for pantothenate biosynthesis in Arabidopsis thaliana, pantothenate, i.e. vitamin B5, is the universal precursor for coenzyme A, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FKB3 | ecotype Columbia-0, single gene | - |
Escherichia coli | - |
gene panC | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
seed | transcript and metabolite patterns of CoA biosynthesis during seed development involving the enzyme, overview | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | - |
Escherichia coli | AMP + diphosphate + (R)-pantothenate | - |
? | |
ATP + (R)-pantoate + beta-alanine | - |
Arabidopsis thaliana | AMP + diphosphate + (R)-pantothenate | - |
? | |
additional information | pantothenate synthetase is essential but not limiting for pantothenate biosynthesis in Arabidopsis thaliana, pantothenate, i.e. vitamin B5, is the universal precursor for coenzyme A, overview | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Pantothenate synthetase | - |
Escherichia coli |
Pantothenate synthetase | - |
Arabidopsis thaliana |
PTS | - |
Escherichia coli |
PTS | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Escherichia coli |
9 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli | |
ATP | - |
Arabidopsis thaliana |