Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as N-terminal His-tagged protein | Arabidopsis thaliana |
expression in Escherichia coli BL21 as N-terminal His-tagged protein | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E132A | kcat for beta-alanine is 3.7fold higher than wild-type value, kcat for pantoate is 21fold higher than wild-type value | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-pantoate | substrate inhibition | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | Escherichia coli | synthesis of pantothenate, the essential precursor to coenzyme A | AMP + diphosphate + (R)-pantothenate | - |
? | |
ATP + (R)-pantoate + beta-alanine | Arabidopsis thaliana | synthesis of pantothenate, the essential precursor to coenzyme A | AMP + diphosphate + (R)-pantothenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | synthesis of pantothenate, the essential precursor to coenzyme A | Escherichia coli | AMP + diphosphate + (R)-pantothenate | - |
? | |
ATP + (R)-pantoate + beta-alanine | synthesis of pantothenate, the essential precursor to coenzyme A | Arabidopsis thaliana | AMP + diphosphate + (R)-pantothenate | - |
? | |
ATP + (R)-pantoate + beta-alanine | Bi Uni Uni Bi kinetic mechanism. Enzyme displays non-allosteric behavior | Escherichia coli | AMP + diphosphate + (R)-pantothenate | - |
? | |
ATP + (R)-pantoate + beta-alanine | the two-step reaction mechanism, large allosteric effects | Arabidopsis thaliana | AMP + diphosphate + (R)-pantothenate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
dimer | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
(R)-pantoate | 25°C, pH 8.0, wild-type enzyme | Arabidopsis thaliana | |
0.16 | - |
beta-Alanine | 25°C, pH 8.0, wild-type enzyme | Arabidopsis thaliana | |
0.59 | - |
beta-Alanine | 25°C, pH 8.0, mutant enzyme E132A | Arabidopsis thaliana | |
0.81 | - |
(R)-pantoate | 25°C, pH 8.0, mutant enzyme E132A | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
exchange between beta-alanine and pantothenate in the presence of AMP | Escherichia coli |
5.5 | - |
exchange between beta-alanine and pantothenate in the presence of AMP | Arabidopsis thaliana |
9 | - |
synthesis of pantothenate | Escherichia coli |
9 | - |
synthesis of pantothenate | Arabidopsis thaliana |