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Literature summary for 6.3.2.1 extracted from

  • Jonczyk, R.; Genschel, U.
    Molecular adaptation and allostery in plant pantothenate synthetases (2006), J. Biol. Chem., 281, 37435-37446.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as N-terminal His-tagged protein Arabidopsis thaliana
expression in Escherichia coli BL21 as N-terminal His-tagged protein Escherichia coli

Protein Variants

Protein Variants Comment Organism
E132A kcat for beta-alanine is 3.7fold higher than wild-type value, kcat for pantoate is 21fold higher than wild-type value Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
(R)-pantoate substrate inhibition Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-pantoate + beta-alanine Escherichia coli synthesis of pantothenate, the essential precursor to coenzyme A AMP + diphosphate + (R)-pantothenate
-
?
ATP + (R)-pantoate + beta-alanine Arabidopsis thaliana synthesis of pantothenate, the essential precursor to coenzyme A AMP + diphosphate + (R)-pantothenate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli
-
Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantoate + beta-alanine synthesis of pantothenate, the essential precursor to coenzyme A Escherichia coli AMP + diphosphate + (R)-pantothenate
-
?
ATP + (R)-pantoate + beta-alanine synthesis of pantothenate, the essential precursor to coenzyme A Arabidopsis thaliana AMP + diphosphate + (R)-pantothenate
-
?
ATP + (R)-pantoate + beta-alanine Bi Uni Uni Bi kinetic mechanism. Enzyme displays non-allosteric behavior Escherichia coli AMP + diphosphate + (R)-pantothenate
-
?
ATP + (R)-pantoate + beta-alanine the two-step reaction mechanism, large allosteric effects Arabidopsis thaliana AMP + diphosphate + (R)-pantothenate
-
?

Subunits

Subunits Comment Organism
dimer
-
Escherichia coli
dimer
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.039
-
(R)-pantoate 25°C, pH 8.0, wild-type enzyme Arabidopsis thaliana
0.16
-
beta-Alanine 25°C, pH 8.0, wild-type enzyme Arabidopsis thaliana
0.59
-
beta-Alanine 25°C, pH 8.0, mutant enzyme E132A Arabidopsis thaliana
0.81
-
(R)-pantoate 25°C, pH 8.0, mutant enzyme E132A Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
exchange between beta-alanine and pantothenate in the presence of AMP Escherichia coli
5.5
-
exchange between beta-alanine and pantothenate in the presence of AMP Arabidopsis thaliana
9
-
synthesis of pantothenate Escherichia coli
9
-
synthesis of pantothenate Arabidopsis thaliana