Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes expressed in Escherichia coli BL21 | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
H44A | greater than 1000fold reduction in enzyme activity | Mycobacterium tuberculosis |
H47A | greater than 1000fold reduction in enzyme activity | Mycobacterium tuberculosis |
K160A | greater than 1000fold reduction in enzyme activity | Mycobacterium tuberculosis |
K160C | activity is markedly enhanced by the alkylation of cysteine with bromoethylamine | Mycobacterium tuberculosis |
N69A | greater than 1000fold reduction in enzyme activity | Mycobacterium tuberculosis |
Q164A | mutant exhibits 50fold less activity than wild-type enzyme | Mycobacterium tuberculosis |
Q72A | greater than 1000fold reduction in enzyme activity | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
beta-Alanine | 25°C, pH 7.8, mutant enzyme K160C | Mycobacterium tuberculosis | |
0.27 | - |
beta-Alanine | 25°C, pH 7.8, wild-type enzyme | Mycobacterium tuberculosis | |
0.3 | - |
beta-Alanine | 25°C, pH 7.8, mutant enzyme Q164A | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | - |
Mycobacterium tuberculosis | AMP + diphosphate + (R)-pantothenate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Pantothenate synthetase | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
beta-Alanine | 25°C, pH 7.8, mutant enzyme K160C | Mycobacterium tuberculosis | |
0.02 | - |
beta-Alanine | 25°C, pH 7.8, mutant enzyme Q164A | Mycobacterium tuberculosis | |
1.01 | - |
beta-Alanine | 25°C, pH 7.8, wild-type enzyme | Mycobacterium tuberculosis |