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Literature summary for 6.3.2.1 extracted from

  • Zheng, R.; Dam, T.K.; Brewer, C.F.; Blanchard, J.S.
    Active site residues in Mycobacterium tuberculosis pantothenate synthetase required in the formation and stabilization of the adenylate intermediate (2004), Biochemistry, 43, 7171-7178.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes expressed in Escherichia coli BL21 Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
H44A greater than 1000fold reduction in enzyme activity Mycobacterium tuberculosis
H47A greater than 1000fold reduction in enzyme activity Mycobacterium tuberculosis
K160A greater than 1000fold reduction in enzyme activity Mycobacterium tuberculosis
K160C activity is markedly enhanced by the alkylation of cysteine with bromoethylamine Mycobacterium tuberculosis
N69A greater than 1000fold reduction in enzyme activity Mycobacterium tuberculosis
Q164A mutant exhibits 50fold less activity than wild-type enzyme Mycobacterium tuberculosis
Q72A greater than 1000fold reduction in enzyme activity Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
beta-Alanine 25°C, pH 7.8, mutant enzyme K160C Mycobacterium tuberculosis
0.27
-
beta-Alanine 25°C, pH 7.8, wild-type enzyme Mycobacterium tuberculosis
0.3
-
beta-Alanine 25°C, pH 7.8, mutant enzyme Q164A Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantoate + beta-alanine
-
Mycobacterium tuberculosis AMP + diphosphate + (R)-pantothenate
-
?

Synonyms

Synonyms Comment Organism
Pantothenate synthetase
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
beta-Alanine 25°C, pH 7.8, mutant enzyme K160C Mycobacterium tuberculosis
0.02
-
beta-Alanine 25°C, pH 7.8, mutant enzyme Q164A Mycobacterium tuberculosis
1.01
-
beta-Alanine 25°C, pH 7.8, wild-type enzyme Mycobacterium tuberculosis