Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
crystals of native PS and PS complexed with alpha,beta-methyleneadenosine 5'-triphosphate, pantoate and the reaction intermediate pantoyl adenylate, crystals are grown by hanging-drop vapor diffusion, 0.003-0.005 ml of PS solution at 10 mg/ml PS is mixed with an equal volume of well solution, best crystals are obtained with well solutions containing 10%-15% polyethylene glycol 3000, 5% glycerol, 2% ethanol, 20 mM MgCl2, 150 mM Li2SO4 and 100 mM imidazole, pH 8.0 at 20°C, crystals diffract to 1.6-2.0 A | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
E77G | 87% of wild-type activity | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31611 | - |
2 * 31611, mass spectroscopy, crystallization | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | Mycobacterium tuberculosis | - |
AMP + diphosphate + (R)-pantothenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant PS | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | - |
Mycobacterium tuberculosis | AMP + diphosphate + (R)-pantothenate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31611, mass spectroscopy, crystallization | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
panthotenate synthetase | - |
Mycobacterium tuberculosis |
PS | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.74 | - |
(R)-pantoate | - |
Mycobacterium tuberculosis | |
2.94 | - |
(R)-pantoate | - |
Mycobacterium tuberculosis |