Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.1 extracted from

  • Zheng, R.; Blanchard, J.S.
    Steady-state and pre-steady-state kinetic analysis of Mycobacterium tuberculosis pantothenate synthetase (2001), Biochemistry, 40, 12904-12912.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
pantothenate product inhibition Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
D-pantoate 25°C, pH 7.8 Mycobacterium tuberculosis
0.8
-
beta-Alanine 25°C, pH 7.8 Mycobacterium tuberculosis
2.6
-
ATP 25°C, pH 7.8 Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ maximal activity at 10 mM, slight activation Mycobacterium tuberculosis
Mg2+ maximal activity at 10 mM, very strong activation Mycobacterium tuberculosis
Mn2+ maximal activity at 10 mM, very strong activation Mycobacterium tuberculosis
Ni2+ maximal activity at approx. 3 mM, slight activation Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32677
-
2 * 32677, deduced from nucleotide sequence Mycobacterium tuberculosis
33000
-
2 * 33000, SDS-PAGE Mycobacterium tuberculosis
65000
-
gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-pantoate + beta-alanine Mycobacterium tuberculosis last step in pantothenate biosynthesis pathway AMP + diphosphate + (R)-pantothenate
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
PanC is essential for growth
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PanC Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantoate + beta-alanine
-
Mycobacterium tuberculosis AMP + diphosphate + (R)-pantothenate
-
?
ATP + (R)-pantoate + beta-alanine last step in pantothenate biosynthesis pathway Mycobacterium tuberculosis AMP + diphosphate + (R)-pantothenate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 33000, SDS-PAGE Mycobacterium tuberculosis
dimer 2 * 32677, deduced from nucleotide sequence Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
PanC
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4
-
beta-Alanine 25°C, pH 7.8 Mycobacterium tuberculosis
3.4
-
D-pantoate 25°C, pH 7.8 Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.5
-
pantothenate 25°C, pH 7.8, vs. beta-alanine Mycobacterium tuberculosis
5.7
-
pantothenate 25°C, pH 7.8, vs. D-pantoate Mycobacterium tuberculosis
7.6
-
pantothenate 25°C, pH 7.8, vs. ATP Mycobacterium tuberculosis