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Literature summary for 6.3.1.5 extracted from

  • Wojcik, M.; Seidle, H.F.; Bieganowski, P.; Brenner, C.
    Glutamine-dependent NAD+ synthetase. How a two-domain, three-substrate enzyme avoids waste (2006), J. Biol. Chem., 281, 33395-33402.
    View publication on PubMed
Search for more literature for 6.3.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D593A poor synthetase activity Saccharomyces cerevisiae
D593A/F622A synthetase dead Saccharomyces cerevisiae
E177A poor synthetase activity Saccharomyces cerevisiae
F622A poor synthetase activity Saccharomyces cerevisiae
I111A poor synthetase activity Saccharomyces cerevisiae
L529A/L604A poor synthetase activity Saccharomyces cerevisiae
L604A poor synthetase activity Saccharomyces cerevisiae
L604N poor synthetase activity Saccharomyces cerevisiae
M621A synthetase mutant that depresses all activities Saccharomyces cerevisiae
R112L poor synthetase activity that inhibit substrate synergism Saccharomyces cerevisiae
R112S poor synthetase activity that inhibit substrate synergism Saccharomyces cerevisiae
Y532A/Y601A synthetase dead Saccharomyces cerevisiae
Y601A poor synthetase activity Saccharomyces cerevisiae
Y601A/M621A synthetase dead Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0003
-
 mutant enzyme R112L, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin Saccharomyces cerevisiae
0.0011
-
 mutant enzyme D593A/F622A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin Saccharomyces cerevisiae
0.0027
-
 mutant enzyme L604A/L519A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin Saccharomyces cerevisiae
0.0033
-
 mutant enzyme Y532A/M621A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin Saccharomyces cerevisiae
0.0043
-
 mutant enzyme L604N, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin Saccharomyces cerevisiae
0.008
-
 mutant enzyme F622A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin Saccharomyces cerevisiae
0.0143
-
 mutant enzyme I111A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin Saccharomyces cerevisiae
0.015
-
 mutant enzyme R112S, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin Saccharomyces cerevisiae
0.0485
-
 mutant enzyme Y601A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin Saccharomyces cerevisiae
0.152
-
 mutant enzyme D593A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin Saccharomyces cerevisiae
0.183
-
 mutant enzyme L604A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin Saccharomyces cerevisiae
0.25
-
 mutant enzyme M621A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin Saccharomyces cerevisiae
0.631
-
 mutant enzyme E177A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin Saccharomyces cerevisiae
0.741
-
 wild type enzyme, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + NH3
-
 Saccharomyces cerevisiae AMP + diphosphate + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
NH3-dependent NAD+ synthetase
-
 Saccharomyces cerevisiae
Qns1 couples a glutamine amidotransferase domain to a second active site that requires ammonia as a reactant Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae