Cloned (Comment) | Organism |
---|---|
nadE gene, expression of the enzyme as a His-tagged protein and as a thioredoxin-His6-fusion protein in insect cells of Thrichoplusia ni BTI-TN-5B1-4 via baculovirus infection, for large scale production, and in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
thrombin | enzyme is highly susceptible too proteolytic cleavage | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00052 | - |
deamido-NAD+ | pH 8.5, 37°C, recombinant truncated enzyme | Mycobacterium tuberculosis | |
0.00079 | - |
ATP | pH 8.5, 37°C, recombinant truncated enzyme | Mycobacterium tuberculosis | |
0.0011 | - |
glutamine | pH 8.5, 37°C, recombinant full length enzyme | Mycobacterium tuberculosis | |
0.0013 | - |
ATP | pH 8.5, 37°C, recombinant full length enzyme | Mycobacterium tuberculosis | |
0.0016 | - |
glutamine | pH 8.5, 37°C, recombinant truncated enzyme | Mycobacterium tuberculosis | |
0.0029 | - |
deamido-NAD+ | pH 8.5, 37°C, recombinant full length enzyme | Mycobacterium tuberculosis | |
0.025 | - |
NH4+ | pH 8.5, 37°C, recombinant truncated enzyme | Mycobacterium tuberculosis | |
0.027 | - |
NH4+ | pH 8.5, 37°C, recombinant full length enzyme | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + NH4+ | Mycobacterium tuberculosis | - |
AMP + diphosphate + NAD+ + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme and recombinant thioredoxin-His6-fusion protein from Escherichia coli and insect cells, the His-tag is cleaved off afterwards | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.009 | - |
partially purified recombinant His-tagged enzyme expressed in insect cells, substrate NH4+ | Mycobacterium tuberculosis |
0.033 | - |
purified recombinant HIs-tagged enzyme expressed in Escherichia coli, substrate glutamine | Mycobacterium tuberculosis |
0.1 | - |
purified recombinant His-tagged enzyme expressed in Escherichia coli, substrate NH4+ | Mycobacterium tuberculosis |
0.67 | - |
purified recombinant His-tagged truncated enzyme expressed in Escherichia coli, substrate NH4+ | Mycobacterium tuberculosis |
Storage Stability | Organism |
---|---|
-20°C, recombinant truncated enzyme, overnight, loss of 59% activity | Mycobacterium tuberculosis |
-70°C, recombinant truncated enzyme, overnight, loss of 30% activity | Mycobacterium tuberculosis |
-80 - 4°C, recombinant full length enzyme, several months | Mycobacterium tuberculosis |
25°C, recombinant truncated enzyme, stable | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + glutamine | - |
Mycobacterium tuberculosis | AMP + diphosphate + NAD+ + glutamate | - |
? | |
ATP + deamido-NAD+ + NH4+ | - |
Mycobacterium tuberculosis | AMP + diphosphate + NAD+ + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD synthase | - |
Mycobacterium tuberculosis |
NAD synthetase | - |
Mycobacterium tuberculosis |
Nicotinamide adenine dinucleotide synthetase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
with substrate NH4+ | Mycobacterium tuberculosis |
8.8 | - |
with substrate glutamine | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | required | Mycobacterium tuberculosis |