Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.1.5 extracted from

  • Symersky, J.; Devedjiev, Y.; Moore, K.; Brouillette, C.; DeLucas, L.
    NH3-dependent NAD+ synthetase from Bacillus subtilis at 1 A resolution (2002), Acta Crystallogr. Sect. D, 58, 1138-1146.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme complexed with substrates, vapour diffusion method at 5.2 under earth gravity at pH 5.2, and microseeding under space microgravity, the latter in protein solution containing 15 mg/ml protein, 2.5 mM ATP, 5 mM deamido-NAD+, 50 mM Tris, pH 8.5, 50 mM MgCl2, 25% PEG 400 v/v, 9 days, X-ray diffractio structure determination at 1.0 A resolution and analysis of the crystal structures at pH 5.2 and pH 8.5 Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deamido-NAD+ + NH3 Bacillus subtilis
-
AMP + diphosphate + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P08164 purified recombinant enzyme
-

Reaction

Reaction Comment Organism Reaction ID
ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+ mechanism of two-step procedure, reaction intermediate Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + NH3
-
Bacillus subtilis AMP + diphosphate + NAD+
-
?

Synonyms

Synonyms Comment Organism
NAD synthase
-
Bacillus subtilis
NAD synthetase
-
Bacillus subtilis
Nicotinamide adenine dinucleotide synthetase
-
Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Bacillus subtilis