Cloned (Comment) | Organism |
---|---|
expression of isozyme MtGS1a in Escherichia coli | Medicago truncatula |
Protein Variants | Comment | Organism |
---|---|---|
Y167F | site-directed mutagenesis of isozyme MtGS1a reduces the inhibition by tetranitromethane | Medicago truncatula |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | inhibits isozyme MtGS2a | Medicago truncatula | |
iodoacetamide | inhibits isozyme MtGS1a | Medicago truncatula | |
additional information | no inhibition by H2O2 and S-nitrosoglutathione, epicatechin is able to protect isozyme MtGS1a from inactivation; no inhibition by iodoacetamide, epicatechin is not able to protect isozyme MtGS2a from inactivation | Medicago truncatula | |
N-Acetylimidazole | inhibition of isozyme MtGS1a; slight inhibition of isozyme MtGS2a | Medicago truncatula | |
peroxynitrite | inhibition of isozyme MtGS1a | Medicago truncatula | |
S-nitrosoglutathione | isozyme MtGS2a activity is inhibited by thiol residue nitrosylation | Medicago truncatula | |
sodium nitroprusside | NO donor sodium nitroprusside results in increased in vivo enzyme nitration accompanied by a reduction in enzyme activity; NO donor sodium nitroprusside results in increased in vivo enzyme nitration accompanied by a reduction in enzyme activity | Medicago truncatula | |
Tetranitromethane | inhibition of isozyme MtGS1a | Medicago truncatula |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isozyme MtGS2a | Medicago truncatula | 9507 | - |
cytosol | isoenzyme MtGS1a | Medicago truncatula | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + NH3 | Medicago truncatula | - |
ADP + phosphate + L-glutamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago truncatula | O04998 | MtGS1a; isozyme MtGS1a | - |
Medicago truncatula | O04999 | MtGS2a; isozymes MtGS1a and MtGS2a | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
nitrosylation | isozyme MtGS2a activity is inhibited by thiol residue nitrosylation | Medicago truncatula |
side-chain modification | the enzyme is regulated by tyrosine nitration. The enzyme is nitrated in planta and its nitration status changes in relation to active nitrogen fixation | Medicago truncatula |
Purification (Comment) | Organism |
---|---|
recombinant isozyme MtGS1a from Escherichia coli | Medicago truncatula |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | root nodule, isozyme MtGS1a | Medicago truncatula | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + NH3 | - |
Medicago truncatula | ADP + phosphate + L-glutamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Glutamine synthetase | - |
Medicago truncatula |
General Information | Comment | Organism |
---|---|---|
physiological function | glutamine synthetase is a key enzyme for root nodule metabolism, and is a molecular target of nitric oxide in root nodules of Medicago truncatula and is regulated by tyrosine nitration. NO-mediated GS posttranslational inactivation is related to metabolite channeling to boost the nodule antioxidant defenses in response to NO | Medicago truncatula |