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Literature summary for 6.3.1.2 extracted from

  • van Rooyen, J.; Belrhali, H.; Abratt, V.; Sewell, B.T.
    Proteolysis of the type III glutamine synthetase from Bacteroides fragilis causes expedient crystal-packing rearrangements (2011), Acta Crystallogr. Sect. F, 67, 358-363.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis rapid and scalable two-step protocol for expression and purification of glutamine synthetase in an auxotrophic Escherichia coli strain utilizing differential precipitation by divalent cations followed by affinity chromatography to produce suitable quantities of homogenous material for structural characterization Bacteroides fragilis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacteroides fragilis

Crystallization (Commentary)

Crystallization (Comment) Organism
untagged, full-length recombinant protein, to 3.0 A resolution, space group P1 Bacteroides fragilis

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis Q5LGP1
-
-

Purification (Commentary)

Purification (Comment) Organism
rapid and scalable two-step protocol for expression and purification of glutamine synthetase in an auxotrophic Escherichia coli strain utilizing differential precipitation by divalent cations followed by affinity chromatography to produce suitable quantities of homogenous material for structural characterization Bacteroides fragilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11
-
pH not specified in the publication, temperature not specified in the publication Bacteroides fragilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Gln + hydroxylamine + ADP
-
Bacteroides fragilis L-gamma-glutamylhydroxamate + NH4+ + ?
-
?