Application | Comment | Organism |
---|---|---|
synthesis | rapid and scalable two-step protocol for expression and purification of glutamine synthetase in an auxotrophic Escherichia coli strain utilizing differential precipitation by divalent cations followed by affinity chromatography to produce suitable quantities of homogenous material for structural characterization | Bacteroides fragilis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacteroides fragilis |
Crystallization (Comment) | Organism |
---|---|
untagged, full-length recombinant protein, to 3.0 A resolution, space group P1 | Bacteroides fragilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides fragilis | Q5LGP1 | - |
- |
Purification (Comment) | Organism |
---|---|
rapid and scalable two-step protocol for expression and purification of glutamine synthetase in an auxotrophic Escherichia coli strain utilizing differential precipitation by divalent cations followed by affinity chromatography to produce suitable quantities of homogenous material for structural characterization | Bacteroides fragilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
pH not specified in the publication, temperature not specified in the publication | Bacteroides fragilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Gln + hydroxylamine + ADP | - |
Bacteroides fragilis | L-gamma-glutamylhydroxamate + NH4+ + ? | - |
? |