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Literature summary for 6.3.1.2 extracted from

  • Martinez-Espinosa, R.M.; Esclapez, J.; Bautista, V.; Bonete, M.J.
    An octameric prokaryotic glutamine synthetase from the haloarchaeon Haloferax mediterranei (2006), FEMS Microbiol. Lett., 264, 110-116.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
methionine sulfoximine 2.5 mM causes up to 60% inhibition of the transferase GS activity and in the presence of 6 mM, the transferase GS activity is around 20% of the controls Haloferax mediterranei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
ATP at 45°C Haloferax mediterranei
4.9
-
L-Glu at 45°C Haloferax mediterranei
10.5
-
hydroxylamine at 45°C Haloferax mediterranei
25
-
L-Gln at 45°C Haloferax mediterranei

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates activity at 1 mM Haloferax mediterranei
Co2+ stimulates activity at 1 mM Haloferax mediterranei
Cu2+ stimulates activity at 1 mM Haloferax mediterranei
Fe2+ stimulates activity at 1 mM Haloferax mediterranei
K+ 0.25 mM as optimal concentration for highest activity Haloferax mediterranei
Mg2+ stimulates activity at 1 mM Haloferax mediterranei
Mn2+ most effective cation for stimulation of activity at 1 mM Haloferax mediterranei
Na+ 0.5 mM as optimal concentration for highest activity Haloferax mediterranei
Ni2+ stimulates activity at 1 mM Haloferax mediterranei
Zn2+ stimulates activity at 1 mM Haloferax mediterranei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51700
-
8 * 51700, gel filtration Haloferax mediterranei
445000
-
gel filtration Haloferax mediterranei

Organism

Organism UniProt Comment Textmining
Haloferax mediterranei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose-4B column chromatography, DEAE-cellulose column chromatography, and Sephacryl S-300 gel filtration Haloferax mediterranei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0002
-
crude cell extract Haloferax mediterranei
0.0149
-
after 75fold purification Haloferax mediterranei

Storage Stability

Storage Stability Organism
4°C, in 0.25 M MOPS buffer with 1.5 M KCl, 50 h, 11% residual activity Haloferax mediterranei
4°C, in 0.25 M MOPS buffer with 1.5 M NaCl, 50 h, 60% residual activity Haloferax mediterranei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
Haloferax mediterranei ADP + phosphate + L-Gln
-
?
L-Gln + hydroxylamine + ADP
-
Haloferax mediterranei gamma-glutamylhydroxamate + NH4+ + ?
-
?

Subunits

Subunits Comment Organism
octamer 8 * 51700, gel filtration Haloferax mediterranei

Synonyms

Synonyms Comment Organism
Glutamine synthetase
-
Haloferax mediterranei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Haloferax mediterranei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60
-
Haloferax mediterranei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Haloferax mediterranei

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Haloferax mediterranei