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Literature summary for 6.3.1.2 extracted from

  • Kahl, S.; Von Berswordt-Wallrabe, P.; Heeschen, V.; Schmidt, H.; Rudolph, H.
    Plastidic L-glutamine synthetase. A membrane-bound enzyme in Sphagnum fallax (1998), J. Plant Physiol., 153, 270-275.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
NH4+ chloroplast enzyme Sphagnum fallax
3
-
ATP chloroplast enzyme Sphagnum fallax
22
-
L-glutamate chloroplast enzyme Sphagnum fallax

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast isoform 2, reversibly associated to thylakoid membrane, activity not influenced when enzyme is detached from membrane Sphagnum fallax 9507
-
cytosol isoform 1 Sphagnum fallax 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
alpha, beta, 4 * 58000 + 4 * 65000, native mass by gel filtration, subunit mass by SDS-PAGE Sphagnum fallax
65000
-
alpha, beta, 4 * 58000 + 4 * 65000, native mass by gel filtration, subunit mass by SDS-PAGE Sphagnum fallax
492000
-
gel filtration Sphagnum fallax

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + glutamate + NH4+ Sphagnum fallax central enzyme of nitrogen metabolism ADP + phosphate + L-glutamine
-
?

Organism

Organism UniProt Comment Textmining
Sphagnum fallax
-
two isoforms
-

Purification (Commentary)

Purification (Comment) Organism
-
Sphagnum fallax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glutamate + NH4+
-
Sphagnum fallax ADP + phosphate + L-glutamine
-
?
ATP + glutamate + NH4+ central enzyme of nitrogen metabolism Sphagnum fallax ADP + phosphate + L-glutamine
-
?

Subunits

Subunits Comment Organism
octamer alpha, beta, 4 * 58000 + 4 * 65000, native mass by gel filtration, subunit mass by SDS-PAGE Sphagnum fallax

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Sphagnum fallax

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8
-
Sphagnum fallax