Cloned (Comment) | Organism |
---|---|
SA0 and SA1 expressed in Escherichia coli SE3000 | Trichormus variabilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alanine | more inhibitory for enzyme from strain SA0 than for enzyme from SA1 | Trichormus variabilis | |
aspartate | more inhibitory for enzyme from strain SA0 than for enzyme from SA1 | Trichormus variabilis | |
cysteine | more inhibitory for enzyme from strain SA0 than for enzyme from SA1 | Trichormus variabilis | |
glycine | more inhibitory for enzyme from strain SA0 than for enzyme from SA1 | Trichormus variabilis | |
L-methionine sulfone | inhibits enzyme from strain SA0 but not from SA1, more than 80% inhibition at 0.05 mM | Trichormus variabilis | |
L-methionine-DL-sulfoximine | inhibits enzyme from strain SA0 but not from SA1, more than 80% inhibition at 0.05 mM | Trichormus variabilis | |
Mn2+ | inhibitory for enzyme from strain SA0 | Trichormus variabilis | |
serine | more inhibitory for enzyme from strain SA0 than for enzyme from SA1 | Trichormus variabilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NH4+ | enzyme from strain SA0, pH 6.8 | Trichormus variabilis | |
0.015 | - |
NH4+ | enzyme from strain SA0, pH 7.5 | Trichormus variabilis | |
0.1 | - |
ATP | enzyme from strain SA0, pH 6.8 | Trichormus variabilis | |
0.13 | - |
ATP | enzyme from strain SA0, pH 7.5 | Trichormus variabilis | |
0.17 | - |
ATP | enzyme from strain SA1, pH 6.8 | Trichormus variabilis | |
0.21 | - |
ATP | enzyme from strain SA1, pH 7.5 | Trichormus variabilis | |
1.4 | - |
glutamate | enzyme from strain SA0, pH 6.8 | Trichormus variabilis | |
1.4 | - |
NH4+ | enzyme from strain SA1, pH 7.5 | Trichormus variabilis | |
1.7 | - |
glutamate | enzyme from strain SA0, pH 7.5 | Trichormus variabilis | |
9.2 | - |
glutamate | enzyme from strain SA1, pH 6.8 | Trichormus variabilis | |
12.4 | - |
NH4+ | enzyme from strain SA1, pH 6.8 | Trichormus variabilis | |
16.8 | - |
glutamate | enzyme from strain SA1, pH 7.5 | Trichormus variabilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for optimum activity of enzyme from strain SA0, no activation of enzyme from strain SA1 | Trichormus variabilis | |
Mn2+ | required for optimum activity of enzyme from strain SA1, highest activity at 0.3 mM, concentration inhibitory for enzyme from SA0 | Trichormus variabilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
SDS-PAGE | Trichormus variabilis |
53080 | - |
calculated from amino acid sequence | Trichormus variabilis |
625000 | - |
gel filtration | Trichormus variabilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glutamate + NH4+ | Trichormus variabilis | central enzyme of nitrogen metabolism, provides glutamine for biosynthesis, ammonia assimilation | ADP + phosphate + L-glutamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichormus variabilis | - |
strains SA0 and SA1, SA1 carries a single amino acid mutation Y183C and is defective in ammonia assimilation below concentrations of 0.5 mM | - |
Purification (Comment) | Organism |
---|---|
from both strains | Trichormus variabilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.1 | - |
purified enzyme from strain SA1 | Trichormus variabilis |
126.1 | - |
purified enzyme from strain SA0 | Trichormus variabilis |
Storage Stability | Organism |
---|---|
-70°C, concentrated solution in 50% glycerol, 2 months, 4% loss of activity | Trichormus variabilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glutamate + NH4+ | - |
Trichormus variabilis | ADP + phosphate + L-glutamine | - |
? | |
ATP + glutamate + NH4+ | central enzyme of nitrogen metabolism, provides glutamine for biosynthesis, ammonia assimilation | Trichormus variabilis | ADP + phosphate + L-glutamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 12 * 52000, subunit mass by SDS-PAGE, native mass by gel filtration | Trichormus variabilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
strain SA1 | Trichormus variabilis |
7.5 | - |
strain SA0 | Trichormus variabilis |