Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.1.2 extracted from

  • Tumani, H.; Shen, G.Q.; Peter, J.B.
    Purification and immunocharacterization of human brain glutamine synthetase and its detection in cerebrospinal fluid and serum by a sandwich enzyme immunoassay (1995), J. Immunol. Methods, 188, 155-163.
    View publication on PubMed

Application

Application Comment Organism
analysis immunoassay that detects synthetase protein samples where the enzyme has been inactivated by repeated cycles of freezing and thawing, and in serum and cerebrospinal fluid where glutamine synthetase is undetectable by the enzyme activity assay Homo sapiens

General Stability

General Stability Organism
more than 50% loss of activity after one freeze/thaw cycle Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
8 * 44000, SDS-PAGE, composed of three different types of subunits with isoelectric points ranging from 7.0-7.2 Homo sapiens
360000 400000 PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-
cerebrospinal fluid
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
Homo sapiens ADP + phosphate + L-Gln
-
?

Subunits

Subunits Comment Organism
octamer 8 * 44000, SDS-PAGE, composed of three different types of subunits with isoelectric points ranging from 7.0-7.2 Homo sapiens