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Literature summary for 6.3.1.2 extracted from

  • Garcia-Dominguez, M.; Reyes, J.C.; Florencio, F.J.
    Purification and characterization of a new type of glutamine synthetase from cyanobacteria (1997), Eur. J. Biochem., 244, 258-264.
    View publication on PubMed
Search for more literature for 6.3.1.2

Cloned(Commentary)

Cloned (Comment) Organism
glnN gene overexpressed in Escherichia coli Synechocystis sp.

General Stability

General Stability Organism
strongly stabilized in presence of Mn2+ but not with other divalent cations. Maximal stabilization at 1 mM Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
ADP strong, enzyme form GSIII Synechocystis sp.
L-methionine sulfoximine enzyme form GS III Synechocystis sp.
Phosphinothricin
-
 Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 NH4+ enzyme form GSIII Synechocystis sp.
0.43
-
 ATP enzyme form GSIII Synechocystis sp.
0.9
-
 Glu enzyme form GSIII Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 12 * 52000, enzyme form GSI, SDS-PAGE Synechocystis sp.
80000
-
 6 * 80000, enzyme form GSIII, SDS-PAGE Synechocystis sp.
500000
-
 enzyme form GSIII, gel filtration Synechocystis sp.
624000
-
 enzyme form GSI, gel filtration Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
 strain PCC 6803
-
Synechocystis sp.
-
 enzyme form GSI and GSIII encoded by the gene glnA and glnB
-

Purification (Commentary)

Purification (Comment) Organism
enzyme form GSIII Synechocystis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36.8
-
-
 Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Glu + NH4+
-
 Synechocystis sp. ADP + phosphate + L-Gln
-
 ?

Subunits

Subunits Comment Organism
dodecamer 12 * 52000, enzyme form GSI, SDS-PAGE Synechocystis sp.
hexamer 6 * 80000, enzyme form GSIII, SDS-PAGE Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 enzyme form GSI, biosynthetic assay Synechocystis sp.
42
-
 enzyme form GSIII, biosynthetic assay Synechocystis sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 20 min, complete inactivation Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 enzyme form GSI Synechocystis sp.
8.3
-
 enzyme form GSIII, biosynthetic assay Synechocystis sp.