Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine | more potent inhibitor in the reaction with Mg2+ than with Mn2+ | Bifidobacterium bifidum | |
adenosine | more potent inhibitor in the reaction with Mg2+ than with Mn2+ | Bifidobacterium breve | |
adenosine | more potent inhibitor in the reaction with Mg2+ than with Mn2+ | Bifidobacterium pseudolongum | |
ADP | more potent inhibition in the reaction with Mg2+ than with Mn2+ | Bifidobacterium bifidum | |
ADP | more potent inhibition in the reaction with Mg2+ than with Mn2+ | Bifidobacterium breve | |
ADP | more potent inhibition in the reaction with Mg2+ than with Mn2+ | Bifidobacterium pseudolongum | |
Ala | more inhibition by L-Ala and glycine in the mixture with Mn2+ than with Mg2+ | Bifidobacterium bifidum | |
Ala | more inhibition by L-Ala and glycine in the mixture with Mn2+ than with Mg2+ | Bifidobacterium breve | |
Ala | more inhibition by L-Ala and glycine in the mixture with Mn2+ than with Mg2+ | Bifidobacterium pseudolongum | |
AMP | more potent inhibitor in the reaction with Mg2+ than with Mn2+ | Bifidobacterium bifidum | |
AMP | more potent inhibitor in the reaction with Mg2+ than with Mn2+ | Bifidobacterium breve | |
AMP | more potent inhibitor in the reaction with Mg2+ than with Mn2+ | Bifidobacterium pseudolongum | |
ATP | ATP in excess of Mg2+ | Bifidobacterium breve | |
ATP | ATP in excess of Mg2+ | Bifidobacterium pseudolongum | |
Ca2+ | - |
Bifidobacterium bifidum | |
Ca2+ | - |
Bifidobacterium pseudolongum | |
Cu2+ | - |
Bifidobacterium bifidum | |
Cu2+ | - |
Bifidobacterium pseudolongum | |
Fe2+ | - |
Bifidobacterium bifidum | |
Fe2+ | - |
Bifidobacterium pseudolongum | |
Gly | more inhibition by L-Ala and Gly in the mixture with Mn2+ than with Mg2+ | Bifidobacterium bifidum | |
Gly | more inhibition by L-Ala and Gly in the mixture with Mn2+ than with Mg2+ | Bifidobacterium breve | |
Gly | more inhibition by L-Ala and Gly in the mixture with Mn2+ than with Mg2+ | Bifidobacterium pseudolongum | |
Hg2+ | - |
Bifidobacterium bifidum | |
Hg2+ | - |
Bifidobacterium pseudolongum | |
Ni2+ | - |
Bifidobacterium bifidum | |
Ni2+ | - |
Bifidobacterium pseudolongum | |
Pb2+ | - |
Bifidobacterium bifidum | |
Zn2+ | - |
Bifidobacterium bifidum | |
Zn2+ | - |
Bifidobacterium pseudolongum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
NH4+ | Mg2+-stimulated | Bifidobacterium bifidum | |
0.59 | - |
NH4+ | Mg2+-stimulated | Bifidobacterium breve | |
0.69 | - |
NH4+ | Mn2+-stimulated | Bifidobacterium breve | |
0.69 | - |
NH4+ | NH4+, Mg2+-stimulated | Bifidobacterium pseudolongum | |
0.71 | - |
NH4+ | Mn2+-stimulated | Bifidobacterium pseudolongum | |
0.77 | - |
NH4+ | Mn2+-stimulated | Bifidobacterium bifidum | |
1.1 | - |
L-Glu | Mg2+-stimulated | Bifidobacterium bifidum | |
1.3 | - |
L-Glu | Mn2+-stimulated | Bifidobacterium breve | |
1.5 | - |
ATP | Mg2+-stimulated | Bifidobacterium pseudolongum | |
1.7 | - |
ATP | Mg2+-stimulated | Bifidobacterium bifidum | |
2 | - |
ATP | Mg2+-stimulated or Mn2+-stimulated | Bifidobacterium bifidum | |
2.1 | - |
L-Glu | Mn2+-stimulated | Bifidobacterium pseudolongum | |
2.3 | - |
ATP | Mn2+-stimulated | Bifidobacterium breve | |
2.5 | - |
ATP | Mn2+-stimulated | Bifidobacterium pseudolongum | |
18 | - |
L-Glu | Mg2+-stimulated | Bifidobacterium pseudolongum | |
21 | - |
L-Glu | Mg2+-stimulated | Bifidobacterium breve | |
25 | - |
L-Glu | Mg2+-stimulated | Bifidobacterium bifidum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | maximal activity at a ration of ATP to Co2+ of 1.0. The addition of 3 mM Co2+ to the biosynthetic reaction mixtures containing 4 mM Mg2+ results in a 3-7-fold increase in activity | Bifidobacterium breve | |
Co2+ | maximal activity at a ration of ATP to Co2+ of 1.0. The addition of 3 mM Co2+ to the biosynthetic reaction mixtures containing 4 mM Mg2+ results in a 3-7-fold increase in activity | Bifidobacterium pseudolongum | |
Co2+ | maximal activity when Co2+ is in excess of ATP | Bifidobacterium bifidum | |
Co2+ | divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective | Bifidobacterium bifidum | |
Co2+ | divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective | Bifidobacterium breve | |
Co2+ | divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective | Bifidobacterium pseudolongum | |
Mg2+ | divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal when Mg2+ is in excess of ATP | Bifidobacterium bifidum | |
Mg2+ | divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal when Mg2+ is in excess of ATP | Bifidobacterium breve | |
Mg2+ | divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal when Mg2+ is in excess of ATP | Bifidobacterium pseudolongum | |
Mn2+ | divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal at a ratio of ATP to Mn2+ of 1.0 | Bifidobacterium bifidum | |
Mn2+ | divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal at a ratio of ATP to Mn2+ of 1.0 | Bifidobacterium breve | |
Mn2+ | divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal at a ratio of ATP to Mn2+ of 1.0 | Bifidobacterium pseudolongum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
500000 | - |
gel filtration | Bifidobacterium bifidum |
500000 | - |
gel filtration | Bifidobacterium breve |
500000 | - |
gel filtration | Bifidobacterium pseudolongum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium bifidum | - |
A | - |
Bifidobacterium bifidum A | - |
A | - |
Bifidobacterium breve | - |
- |
- |
Bifidobacterium breve 203 | - |
- |
- |
Bifidobacterium pseudolongum | - |
A | - |
Bifidobacterium pseudolongum A | - |
A | - |
Purification (Comment) | Organism |
---|---|
- |
Bifidobacterium bifidum |
- |
Bifidobacterium breve |
- |
Bifidobacterium pseudolongum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
73.2 | - |
gamma-glutamyl transferase assay | Bifidobacterium breve |
99.6 | - |
gamma-glutamyl transferase assay | Bifidobacterium pseudolongum |
221.3 | - |
gamma-glutamyl transferase assay | Bifidobacterium bifidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Glu + ethylamine | slightly effective | Bifidobacterium bifidum | ADP + phosphate + ? | - |
? | |
ATP + L-Glu + ethylamine | slightly effective | Bifidobacterium breve | ADP + phosphate + ? | - |
? | |
ATP + L-Glu + ethylamine | slightly effective | Bifidobacterium pseudolongum | ADP + phosphate + ? | - |
? | |
ATP + L-Glu + ethylamine | slightly effective | Bifidobacterium bifidum A | ADP + phosphate + ? | - |
? | |
ATP + L-Glu + ethylamine | slightly effective | Bifidobacterium breve 203 | ADP + phosphate + ? | - |
? | |
ATP + L-Glu + ethylamine | slightly effective | Bifidobacterium pseudolongum A | ADP + phosphate + ? | - |
? | |
ATP + L-Glu + hydroxylamine | 13% of the activity relative to NH4+ | Bifidobacterium breve | ADP + phosphate + gamma-glutamyl hydroxamate | - |
? | |
ATP + L-Glu + hydroxylamine | 59% of the activity relative to NH4+ | Bifidobacterium bifidum | ADP + phosphate + gamma-glutamyl hydroxamate | - |
? | |
ATP + L-Glu + hydroxylamine | 10% of the activity relative to NH4+ | Bifidobacterium pseudolongum | ADP + phosphate + gamma-glutamyl hydroxamate | - |
? | |
ATP + L-Glu + hydroxylamine | 59% of the activity relative to NH4+ | Bifidobacterium bifidum A | ADP + phosphate + gamma-glutamyl hydroxamate | - |
? | |
ATP + L-Glu + hydroxylamine | 13% of the activity relative to NH4+ | Bifidobacterium breve 203 | ADP + phosphate + gamma-glutamyl hydroxamate | - |
? | |
ATP + L-Glu + hydroxylamine | 10% of the activity relative to NH4+ | Bifidobacterium pseudolongum A | ADP + phosphate + gamma-glutamyl hydroxamate | - |
? | |
ATP + L-Glu + methylamine | slightly effective | Bifidobacterium bifidum | ADP + phosphate + ? | - |
? | |
ATP + L-Glu + methylamine | slightly effective | Bifidobacterium breve | ADP + phosphate + ? | - |
? | |
ATP + L-Glu + methylamine | slightly effective | Bifidobacterium pseudolongum | ADP + phosphate + ? | - |
? | |
ATP + L-Glu + methylamine | slightly effective | Bifidobacterium bifidum A | ADP + phosphate + ? | - |
? | |
ATP + L-Glu + methylamine | slightly effective | Bifidobacterium breve 203 | ADP + phosphate + ? | - |
? | |
ATP + L-Glu + methylamine | slightly effective | Bifidobacterium pseudolongum A | ADP + phosphate + ? | - |
? | |
ATP + L-Glu + NH4+ | - |
Bifidobacterium bifidum | ADP + phosphate + L-Gln | - |
? | |
ATP + L-Glu + NH4+ | - |
Bifidobacterium breve | ADP + phosphate + L-Gln | - |
? | |
ATP + L-Glu + NH4+ | - |
Bifidobacterium pseudolongum | ADP + phosphate + L-Gln | - |
? | |
Gln + hydroxylamine + ADP | - |
Bifidobacterium bifidum | gamma-Glutamylhydroxamate + NH4+ + ? | - |
? | |
Gln + hydroxylamine + ADP | - |
Bifidobacterium breve | gamma-Glutamylhydroxamate + NH4+ + ? | - |
? | |
Gln + hydroxylamine + ADP | - |
Bifidobacterium pseudolongum | gamma-Glutamylhydroxamate + NH4+ + ? | - |
? | |
Gln + hydroxylamine + ADP | - |
Bifidobacterium bifidum A | gamma-Glutamylhydroxamate + NH4+ + ? | - |
? | |
Gln + hydroxylamine + ADP | - |
Bifidobacterium breve 203 | gamma-Glutamylhydroxamate + NH4+ + ? | - |
? | |
Gln + hydroxylamine + ADP | - |
Bifidobacterium pseudolongum A | gamma-Glutamylhydroxamate + NH4+ + ? | - |
? | |
GTP + L-Glu + NH4+ | no activity | Bifidobacterium breve | GDP + phosphate + L-Gln | - |
? | |
GTP + L-Glu + NH4+ | no activity | Bifidobacterium pseudolongum | GDP + phosphate + L-Gln | - |
? | |
GTP + L-Glu + NH4+ | 15% of the activity relative to ATP | Bifidobacterium bifidum | GDP + phosphate + L-Gln | - |
? | |
GTP + L-Glu + NH4+ | 15% of the activity relative to ATP | Bifidobacterium bifidum A | GDP + phosphate + L-Gln | - |
? | |
GTP + L-Glu + NH4+ | no activity | Bifidobacterium breve 203 | GDP + phosphate + L-Gln | - |
? | |
GTP + L-Glu + NH4+ | no activity | Bifidobacterium pseudolongum A | GDP + phosphate + L-Gln | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 5.5, 10 min, 14% loss of activity | Bifidobacterium bifidum |
60 | - |
pH 6.0, 10 min, 50% loss of activity | Bifidobacterium breve |
60 | - |
pH 6.0, 10 min, 68% loss of activity | Bifidobacterium pseudolongum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | biosynthetic reaction, with 30 mM Mg2+ | Bifidobacterium bifidum |
6 | 6.5 | biosynthetic reaction, with 30 mM Mg2+ | Bifidobacterium breve |
6 | 6.5 | biosynthetic reaction, with 30 mM Mg2+ | Bifidobacterium pseudolongum |
6.5 | - |
gamma-glutamylhydroxamate forming reaction | Bifidobacterium bifidum |
6.5 | - |
gamma-glutamylhydroxamate forming reaction | Bifidobacterium breve |
6.5 | - |
gamma-glutamylhydroxamate forming reaction | Bifidobacterium pseudolongum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | most stable in this range | Bifidobacterium bifidum |
5.5 | - |
60°C, 10 min, 0.1 M acetate buffer, 14% loss of activity | Bifidobacterium bifidum |
6 | - |
60°C, 10 min, 58% loss of activity | Bifidobacterium pseudolongum |
6 | - |
60°C, 10 min, 0.1 M imidazole-HCl buffer, 50% loss of activity | Bifidobacterium breve |