Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | specific Gate-domain residues are essential for activity, detailed overview | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
ACSL6 isoforms and truncated constructs, splicing variants, sequence organization, overview, expression of His-tagged full-length ACSL6 isoform 2 (F-Gate) and DELTAN-truncated version of isoform 1 (DELTAN-(Y-Gate)) in Escherichia coli strain BL21(DE3) membranes | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F319A | site-directed mutagenesis | Homo sapiens |
F319W | site-directed mutagenesis | Homo sapiens |
F319Y | site-directed mutagenesis | Homo sapiens |
H316L | site-directed mutagenesis | Homo sapiens |
L316H | site-directed mutagenesis | Homo sapiens |
additional information | mutants of full-length ACSL6 isoform 2 (F-Gate) and DELTAN-truncated version of isoform 1 (DELTAN-(Y-Gate)) are obtained by site-directed mutagenesis of the H/L residue pair at position 316 and of the F/Y residue pair at position 319. An ACSL6 construct with a tag at its C-terminus has a far lower activity than a construct with a tag fused to its N-terminus | Homo sapiens |
Y319A | site-directed mutagenesis | Homo sapiens |
Y319F | site-directed mutagenesis | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | an ACSL6 construct with a tag at its C-terminus has a far lower activity than a construct with a tag fused to its N-terminus | Homo sapiens | |
Triton X-100 | Inhibition of acyl-CoA formation, inhibits ACSL6 isoform 2 (F-Gate) and its N-terminus truncated version, DELTAN-(F-Gate) | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | isozyme ACSL6 | Homo sapiens | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acylate + CoA | Homo sapiens | - |
AMP + diphosphate + acyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme ACSL6 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acylate + CoA | - |
Homo sapiens | AMP + diphosphate + acyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACSL6 | - |
Homo sapiens |
Acyl-CoA synthetase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | long-chain acyl-CoA synthetase member 6, ASCL6, is a form present in the plasma membrane of cells. Splicing events affecting the N-terminus and alternative motifs near the ATP-binding site generate different isoforms of ACSL6. Alternative fatty acid Gate-domain motifs are essential determinants for the activity of the human ACSL6 isoforms, which appear to act as homodimeric enzyme as well as in complex with other spliced forms. The diversity of these enzyme species can produce the variety of acyl-CoA synthetase activities that are necessary to generate and repair the hundreds of lipid species present in membranes. Oligomeric complex fomations and interactions between isoforms, overview. The N-terminal domain is not essential for oligomer formation | Homo sapiens |