Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanothermobacter thermautotrophicus |
Protein Variants | Comment | Organism |
---|---|---|
I312A | kcat/Km for acetate is 15fold lower than wild-type value, kcat/Km for propionate is 5.2fold lower than wild-type value. Mutant enzyme shows activity with butyrate | Methanothermobacter thermautotrophicus |
T313K | Km for acetate is 170fold higher than wild-type value, kcat for acetate is 34fold lower than wild-type value | Methanothermobacter thermautotrophicus |
T313V | kcat/Km for acetate is 2.5fold lower than wild-type value, kcat/Km for propionate is identical to wild-type value | Methanothermobacter thermautotrophicus |
V388A | kcat/Km for acetate is 6.9fold lower than wild-type value, kcat/Km for propionate is 2.5fold higher than wild-type value, mutant enzyme shows activity with wild-type enzyme | Methanothermobacter thermautotrophicus |
V388G | kcat/Km for acetate is 93fold lower than wild-type value, kcat/Km for propionate is 26fold lower than wild-type value | Methanothermobacter thermautotrophicus |
W416G | kcat/Km for acetate is 71.5fold lower than wild-type value, kcat/Km for propionate is 13fold lower than wild-type value, mutant enzyme shows activity with: butyrate, valerate, hexanoate, heptanoate, octanoate, 4-methylvalerate and 3-methylvalerate | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
CoA | 65°C, mutant enzyme I312A | Methanothermobacter thermautotrophicus | |
0.18 | - |
CoA | 65°C, mutant enzyme V388G | Methanothermobacter thermautotrophicus | |
0.19 | - |
CoA | 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus | |
0.35 | - |
CoA | 65°C, mutant enzyme V388A | Methanothermobacter thermautotrophicus | |
0.43 | - |
CoA | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
0.46 | - |
Butyrate | 65°C, mutant enzyme T313V | Methanothermobacter thermautotrophicus | |
0.65 | - |
ATP | 65°C, mutant enzyme T313V | Methanothermobacter thermautotrophicus | |
0.7 | - |
CoA | 65°C, mutant enzyme T313V | Methanothermobacter thermautotrophicus | |
2.3 | - |
ATP | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
2.6 | - |
ATP | 65°C, mutant enzyme V388A | Methanothermobacter thermautotrophicus | |
3.3 | - |
ATP | 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus | |
3.4 | - |
ATP | 65°C, mutant enzyme T313K | Methanothermobacter thermautotrophicus | |
3.5 | - |
acetate | 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus | |
4 | - |
ATP | 65°C, mutant enzyme V388G | Methanothermobacter thermautotrophicus | |
4.1 | - |
propionate | 65°C, mutant enzyme V388A | Methanothermobacter thermautotrophicus | |
4.5 | - |
propionate | 65°C, mutant enzyme T313V | Methanothermobacter thermautotrophicus | |
6.1 | - |
hexanoate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
6.6 | - |
ATP | 65°C, mutant enzyme I312A | Methanothermobacter thermautotrophicus | |
7.6 | - |
4-methylvalerate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
10.2 | - |
Heptanoate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
11.1 | - |
valerate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
13.2 | - |
acetate | 65°C, mutant enzyme V388A | Methanothermobacter thermautotrophicus | |
18.1 | - |
Octanoate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
24.6 | - |
acetate | 65°C, mutant enzyme I312A | Methanothermobacter thermautotrophicus | |
29.2 | - |
2-methylvalerate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
36.5 | - |
propionate | 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus | |
39.2 | - |
Butyrate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
128.6 | - |
propionate | 65°C, mutant enzyme V388G | Methanothermobacter thermautotrophicus | |
132.1 | - |
acetate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
151.9 | - |
Butyrate | 65°C, mutant enzyme V388A | Methanothermobacter thermautotrophicus | |
164.4 | - |
acetate | 65°C, mutant enzyme V388G | Methanothermobacter thermautotrophicus | |
188.8 | - |
propionate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
596 | - |
acetate | 65°C, mutant enzyme T313K | Methanothermobacter thermautotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Methanothermobacter thermautotrophicus Z245 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-methylvalerate + CoA | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + 2-methylvaleryl-CoA | - |
? | |
ATP + 3-methylvalerate + CoA | mutant enzyme W416G catalyzes the reaction, no activity with wild-type enzyme | Methanothermobacter thermautotrophicus | AMP + diphosphate + 3-methylvaleryl-CoA | - |
? | |
ATP + 4-methylvalerate + CoA | mutant enzyme W416G catalyzes the reaction, no activity with wild-type enzyme | Methanothermobacter thermautotrophicus | AMP + diphosphate + 4-methylvaleryl-CoA | - |
? | |
ATP + acetate + CoA | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | ATP in form of MgATP2- | Methanothermobacter thermautotrophicus | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | - |
Methanothermobacter thermautotrophicus Z245 | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | ATP in form of MgATP2- | Methanothermobacter thermautotrophicus Z245 | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + butyrate + CoA | mutant enzymes I312A and W416G catalyzes the reaction, no activity with wild-type enzyme | Methanothermobacter thermautotrophicus | AMP + diphosphate + butyryl-CoA | - |
? | |
ATP + butyrate + CoA | mutant enzymes I312A and W416G catalyzes the reaction, no activity with wild-type enzyme | Methanothermobacter thermautotrophicus Z245 | AMP + diphosphate + butyryl-CoA | - |
? | |
ATP + heptanoate + CoA | mutant enzyme W416G catalyzes the reaction, no activity with wild-type enzyme | Methanothermobacter thermautotrophicus | AMP + diphosphate + heptanoyl-CoA | - |
? | |
ATP + hexanoate + CoA | mutant enzyme W416G catalyzes the reaction, no activity with wild-type enzyme | Methanothermobacter thermautotrophicus | AMP + diphosphate + hexanoyl-CoA | - |
? | |
ATP + hexanoate + CoA | mutant enzyme W416G catalyzes the reaction, no activity with wild-type enzyme | Methanothermobacter thermautotrophicus Z245 | AMP + diphosphate + hexanoyl-CoA | - |
? | |
ATP + octanoate + CoA | mutant enzyme W416G catalyzes the reaction, no activity with wild-type enzyme | Methanothermobacter thermautotrophicus | AMP + diphosphate + octanoyl-CoA | - |
? | |
ATP + propionate + CoA | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + propionyl-CoA | - |
? | |
ATP + propionate + CoA | - |
Methanothermobacter thermautotrophicus Z245 | AMP + diphosphate + propionyl-CoA | - |
? | |
ATP + valerate + CoA | mutant enzyme W416G catalyzes the reaction, no activity with wild-type enzyme | Methanothermobacter thermautotrophicus | AMP + diphosphate + valeryl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MT-ACS1 | - |
Methanothermobacter thermautotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
2-methylvalerate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
1.56 | - |
Butyrate | 65°C, mutant enzyme V388A | Methanothermobacter thermautotrophicus | |
1.9 | - |
acetate | 65°C, mutant enzyme T313K | Methanothermobacter thermautotrophicus | |
3.3 | - |
Octanoate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
3.4 | - |
Butyrate | 65°C, mutant enzyme T313V | Methanothermobacter thermautotrophicus | |
5.9 | - |
propionate | 65°C, mutant enzyme T313V | Methanothermobacter thermautotrophicus | |
5.9 | - |
hexanoate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
6 | - |
propionate | 65°C, mutant enzyme V388G | Methanothermobacter thermautotrophicus | |
7.3 | - |
Heptanoate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
10.8 | - |
Butyrate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
10.9 | - |
4-methylvalerate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
11.9 | - |
valerate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
13.2 | - |
propionate | 65°C, mutant enzyme V388A | Methanothermobacter thermautotrophicus | |
19.2 | - |
propionate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
30.4 | - |
acetate | 65°C, mutant enzyme I312A | Methanothermobacter thermautotrophicus | |
31.9 | - |
acetate | 65°C, mutant enzyme V388G | Methanothermobacter thermautotrophicus | |
33.7 | - |
acetate | 65°C, mutant enzyme W416G | Methanothermobacter thermautotrophicus | |
36.2 | - |
acetate | 65°C, mutant enzyme V388A | Methanothermobacter thermautotrophicus | |
46.3 | - |
propionate | 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus | |
65.4 | - |
acetate | 65°C, wild-type enzyme | Methanothermobacter thermautotrophicus |