Cloned (Comment) | Organism |
---|---|
ND-GluRS, phylogenetic analysis | Methanothermobacter thermautotrophicus |
Crystallization (Comment) | Organism |
---|---|
full-length ND-GluRS encompassing residues 1-552, including four cysteines and 18 methionine residues, hanging-drop method by mixing 0.001 ml of reservoir buffer containing 50 mM sodium cacodylate, pH 7.0, 50 mM calcium chloride and 8% PEG 6000, with 0.001 ml of protein solution at 20 mg/ml, 20°C, 5 days, X-ray diffraction structure determination and analysis, single-wavelength anomalous dispersion method, using sulfur anomalous dispersion, modelling | Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63237 | - |
x * 63237, sequence calculation | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGlx | Methanothermobacter thermautotrophicus | - |
AMP + diphosphate + glutamyl-tRNAGlx | - |
? | |
additional information | Methanothermobacter thermautotrophicus | ND-GluRS recognizes both tRNAGlu and tRNAGln without significantly discriminating between them, tRNA discrimination module, overview. The first point of significant distinction between the GlnRS and ND-GluRS tRNA recognition involves residues contacting the G36 nucleobase. A second distiction involves a beta-hairpin module in the CP domain | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGlx | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + glutamyl-tRNAGlx | - |
? | |
additional information | ND-GluRS recognizes both tRNAGlu and tRNAGln without significantly discriminating between them, tRNA discrimination module, overview. The first point of significant distinction between the GlnRS and ND-GluRS tRNA recognition involves residues contacting the G36 nucleobase. A second distiction involves a beta-hairpin module in the CP domain | Methanothermobacter thermautotrophicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63237, sequence calculation | Methanothermobacter thermautotrophicus |
More | structure analysis, and comparison with the structure of the Escherichia coli GlnRS-tRNAGln complex and with GluRS structure, structural determinants responsible for specific tRNAGln recognition, overview | Methanothermobacter thermautotrophicus |
Synonyms | Comment | Organism |
---|---|---|
ND-GluRS | - |
Methanothermobacter thermautotrophicus |
non-discriminating glutamyl-tRNA synthetase | - |
Methanothermobacter thermautotrophicus |
General Information | Comment | Organism |
---|---|---|
evolution | evolution in the GlxRS family, overview. Two pathways exist for the formation of Gln-tRNAGln. The evolutionarily older indirect route utilizes a non-discriminating glutamyl-tRNA synthetase, ND-GluRS, that can form both Glu-tRNAGlu and Glu-tRNAGln. The Glu-tRNAGln is then converted to Gln-tRNAGln by an amidotransferase. ND-GluRS is the evolutionary predecessor of both the glutaminyl-tRNA synthetase, GlnRS, and the eukaryotic discriminating GluRS | Methanothermobacter thermautotrophicus |