Application | Comment | Organism |
---|---|---|
drug development | design of better inhibitors specific for bacterial GluRSs, which are promising targets for antimicrobial therapy | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Glu-AMS | - |
Escherichia coli | |
glutaminyl-beta-ketophosphonate-adenosine | i.e. Gln-KPA, competitive inhibition, non-cognate, binds at one site on the monomeric enzyme | Escherichia coli | |
glutamyl-beta-ketophosphonate-adenosine | i.e. Glu-KPA, selective, competitive inhibition of GluRS, binds at one site on the monomeric enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
1 * 55000, recombinant His6-tagged GluRS, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGlu | Escherichia coli | - |
AMP + diphosphate + L-glutamyl-tRNAGlu | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGlu | - |
Escherichia coli | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
r | |
ATP + L-glutamate + tRNAGlu | a two-step reaction | Escherichia coli | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, recombinant His6-tagged GluRS, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
GluRS | - |
Escherichia coli |
Glutamyl-tRNA synthetase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Escherichia coli | |
0.0000028 | - |
Glu-AMS | pH 7.2, 37°C, versus L-glutamate | Escherichia coli | |
0.018 | - |
glutamyl-beta-ketophosphonate-adenosine | pH 7.2, 37°C, versus L-glutamate | Escherichia coli | |
2.9 | - |
glutaminyl-beta-ketophosphonate-adenosine | pH 7.2, 37°C, versus L-glutamate | Escherichia coli |