Literature summary for 6.1.1.17 extracted from

Simader, H.; Hothorn, M.; Suck, D.
Structures of the interacting domains from yeast glutamyl-tRNA synthetase and tRNA-aminoacylation and nuclear-export cofactor Arc1p reveal a novel function for an old fold (2006), Acta Crystallogr. Sect. D, 62, 1510-1519.

Activating Compound

Activating Compound	Comment	Organism	Structure
Arc1p	accessory protein Arc1p, structure of the interacting domains, crystals structure determination and analysis at 1.9 A resolution, phasing, model building, and refinement of Arc1p, unusual noncrystallographic symmetry in the structure of Arc1p, overview	Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment)	Organism
expression of His6-tagged GluRS-N, comprising residues 1-197, 17-207 and 1-207, in Escherichia coli strain BL21(DE3)	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant truncated enzyme, 0.002 ml of 20 mg/ml protein in 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH 7.2 with NaOH, mixed with 0.002 ml reservoir solution at 20°C, equilibration against 0.075 ml reservoir solution, containing 1.7-1.8 M (NH4)2SO4, 200 mM KSCN for selenomethionine-substituted crystals and 1.8-1.9 M (NH4)2SO4, 200 mM NaI for native crystals, dispersion with selenomethionine, X-ray diffraction structure determination and analysis at 2.5 A resolution, modeling	Saccharomyces cerevisiae

Crystallization (Comment)

Organism

purified recombinant truncated enzyme, 0.002 ml of 20 mg/ml protein in 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH 7.2 with NaOH, mixed with 0.002 ml reservoir solution at 20°C, equilibration against 0.075 ml reservoir solution, containing 1.7-1.8 M (NH4)2SO4, 200 mM KSCN for selenomethionine-substituted crystals and 1.8-1.9 M (NH4)2SO4, 200 mM NaI for native crystals, dispersion with selenomethionine, X-ray diffraction structure determination and analysis at 2.5 A resolution, modeling

Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of truncated enzyme GluRS-N, comprising residues 1-197, 17-207 and 1-207	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Saccharomyces cerevisiae	5737	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-glutamate + tRNAGlu	Saccharomyces cerevisiae	-	AMP + diphosphate + L-glutamyl-tRNAGlu	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P46655	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged GluRS-N from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography and gel filtration	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-glutamate + tRNAGlu	-	Saccharomyces cerevisiae	AMP + diphosphate + L-glutamyl-tRNAGlu	-	?
additional information	GluRS interacts with the accessory protein Arc1p, interaction mode and structure, overview	Saccharomyces cerevisiae	?	-	?

Synonyms

Synonyms	Comment	Organism
GluRS	-	Saccharomyces cerevisiae
Glutamyl-tRNA synthetase	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Saccharomyces cerevisiae