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Literature summary for 6.1.1.17 extracted from
- Higher specific activity of the Escherichia coli glutamyl-tRNA synthetase purified to homogeneity by a six-hour procedure (1992), Protein Expr. Purif., 3, 71-74.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
overproducing strain DH5alpha(pLQ7612) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| six-h procedure | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.95 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C or -70°C, 20 mM sodium Hepes, pH 7.2, 0.1 mM EDTA, 0.5 mM DTT, 65% glycerol, stable for at least 1 year | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamate + tRNAGlu | - |
Escherichia coli | AMP + diphosphate + L-glutamyl-tRNAGlu | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, SDS-PAGE | Escherichia coli |
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Release 2023.1 (January 2023)
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