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Literature summary for 6.1.1.15 extracted from

  • Ambrogelly, A.; Kamtekar, S.; Stathopoulos, C.; Kennedy, D.; Soll, D.
    Asymmetric behavior of archaeal prolyl-tRNA synthetase (2005), FEBS Lett., 579, 6017-6022.
    View publication on PubMed
Search for more literature for 6.1.1.15

Protein Variants

Protein Variants Comment Organism
additional information C-terminal deletion mutant lacking last 50 amino acids, little effect on kinetic parameters Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 tRNAPro pH 7.0, 55°C, C-terminal deletion mutant Methanocaldococcus jannaschii
0.0022
-
 tRNAPro pH 7.0, 55°C, wild-type Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53300
-
 2 * 53300, calcultaed Methanocaldococcus jannaschii
55700
-
 2 * 55700, calculated Methanothermobacter thermautotrophicus
105000
-
 gel filtration Methanocaldococcus jannaschii
113000
-
 gel filtration Methanothermobacter thermautotrophicus

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-
Methanothermobacter thermautotrophicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro one tRNA binds per dimeric enzyme, only 50% of active sites are active Methanothermobacter thermautotrophicus
a
ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro one tRNA binds per dimeric enzyme, only 50% of active sites are active Methanocaldococcus jannaschii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-proline + tRNAPro
-
 Methanothermobacter thermautotrophicus AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
ATP + L-proline + tRNAPro
-
 Methanocaldococcus jannaschii AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 53300, calcultaed Methanocaldococcus jannaschii
dimer 2 * 55700, calculated Methanothermobacter thermautotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 tRNAPro pH 7.0, 55°C, C-terminal deletion mutant Methanocaldococcus jannaschii
1.6
-
 tRNAPro pH 7.0, 55°C, wild-type Methanocaldococcus jannaschii