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Literature summary for 6.1.1.15 extracted from
- Species-specific differences in amino acid editing by class II prolyl-tRNA synthetase (2001), J. Biol. Chem., 276, 30779-30785.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | enzyme is a target for design of antibiotics targeting the editing active site since eukaryotic enzyme types are not able to edit misactivated alanine on tRNAPro | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
L-proline | 60°C, pH not specified in the publication, ATP-diphosphate exchange assay | Methanocaldococcus jannaschii |  |
| 0.18 | - |
L-proline | 37°C | Homo sapiens | |
| 0.25 | - |
L-proline | 37°C | Escherichia coli | |
| 79 | - |
L-alanine | 37°C | Homo sapiens | |
| 140 | - |
L-alanine | 37°C | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-proline + tRNAPro | Escherichia coli | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
| ATP + L-proline + tRNAPro | Homo sapiens | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Methanocaldococcus jannaschii | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-alanine + tRNAPro | very low activity | Homo sapiens | AMP + diphosphate + L-alanyl-tRNAPro | - |
? | |
| ATP + L-alanine + tRNAPro | enzyme possesses both pre- and post-transfer hydrolytic editing activity to prevent from misincorporation of alanine into proteins | Escherichia coli | AMP + diphosphate + L-alanyl-tRNAPro | - |
? | |
| ATP + L-proline + tRNAPro | - |
Escherichia coli | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
| ATP + L-proline + tRNAPro | - |
Escherichia coli | AMP + diphosphate + L-prolyl-tRNAPro | - |
ir | |
| ATP + L-proline + tRNAPro | - |
Homo sapiens | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
| ATP + L-proline + tRNAPro | the enzyme mischarges tRNAPro with alanine at a rate that is 6800fold reduced relative to charging with proline. The enzyme is able to hydrolyze misactivated alanine via both pretransfer and post-transfer editing pathways. Mischarging of a tRNAPro transcript with cysteine is also detected. The enzyme stimulates ATP hydrolysis activity with the noncognate amino acid alanine but not in the presence of proline | Methanocaldococcus jannaschii | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
| additional information | no activity with L-cysteine, the enzyme also performs the ATP-diphosphate exchange reaction, enzyme possesses no editing activity against L-alanine and to prevent from misincorporation of alanine into proteins | Homo sapiens | ? | - |
? | |
| additional information | the enzyme also performs the ATP-diphosphate exchange reaction, no activity with L-cysteine | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Global RNA synthesis factor | - |
Escherichia coli |
| Global RNA synthesis factor | - |
Homo sapiens |
| Pro-tRNA synthetase | - |
Escherichia coli |
| Pro-tRNA synthetase | - |
Homo sapiens |
| Proline translase | - |
Escherichia coli |
| Proline translase | - |
Homo sapiens |
| Proline--tRNA ligase | - |
Escherichia coli |
| Proline--tRNA ligase | - |
Homo sapiens |
| Prolyl RNA synthetase | - |
Escherichia coli |
| Prolyl RNA synthetase | - |
Homo sapiens |
| Prolyl-transfer ribonucleate synthetase | - |
Escherichia coli |
| Prolyl-transfer ribonucleate synthetase | - |
Homo sapiens |
| Prolyl-transfer ribonucleic acid synthetase | - |
Escherichia coli |
| Prolyl-transfer ribonucleic acid synthetase | - |
Homo sapiens |
| Prolyl-transfer RNA synthetase | - |
Escherichia coli |
| Prolyl-transfer RNA synthetase | - |
Homo sapiens |
| Prolyl-tRNA synthetase | - |
Escherichia coli |
| Prolyl-tRNA synthetase | - |
Homo sapiens |
| ProRS | - |
Escherichia coli |
| ProRS | - |
Homo sapiens |
| ProRS | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
| 37 | - |
assay at | Homo sapiens |
| 60 | - |
assay at | Methanocaldococcus jannaschii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
L-alanine | 37°C | Homo sapiens | |
| 1.7 | - |
L-alanine | 37°C | Escherichia coli | |
| 70 | - |
L-proline | 37°C | Escherichia coli | |
| 80 | - |
L-proline | 37°C | Homo sapiens | |
| 210 | - |
L-proline | 60°C, pH not specified in the publication, ATP-diphosphate exchange assay | Methanocaldococcus jannaschii | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
| ATP | - |
Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12000 | - |
L-proline | 60°C, pH not specified in the publication, ATP-diphosphate exchange assay | Methanocaldococcus jannaschii | |
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