Crystallization (Comment) | Organism |
---|---|
structure of the TyrRS-tRNA(Tyr)-L-tyrosine complex, solved at a resolution of 1.95 A | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
D286R | the mutant enzyme aminoacylates the amber suppressor tRNA, as well as the wild-type tRNATyr, whereas the wild-type enzyme aminoacylates the amber suppresssor tRNA about 300fold less efficientyl than the wild-type tRNATyr. The mutant recognizes the amber suppressor tRNA 65fold better than the wild-type enzyme. The activity if the mutant and amber suppressor tRNA pair is as high as 22% that of the wild-type pair. The mutation mainly decreases the KM for tRNA. The kcat is not affected as much | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00035 | - |
tRNATyr(wild-type) | 37°C, pH 7.5, wild-type enzyme | Methanocaldococcus jannaschii | |
0.00068 | - |
tRNATyr(G34C) | 37°C, pH 7.5, mutant enzyme D268R | Methanocaldococcus jannaschii | |
0.0014 | - |
tRNATyr(wild-type) | 37°C, pH 7.5, mutant enzyme D268R | Methanocaldococcus jannaschii | |
0.039 | - |
tRNATyr(G34C) | 37°C, pH 7.5, wild-type enzyme | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + tRNATyr | Methanocaldococcus jannaschii | - |
AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? | |
ATP + L-tyrosine + tRNATyr | Methanocaldococcus jannaschii DSM 2661 | - |
AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q57834 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q57834 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + tRNATyr | - |
Methanocaldococcus jannaschii | AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? | |
ATP + L-tyrosine + tRNATyr | - |
Methanocaldococcus jannaschii DSM 2661 | AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? | |
ATP + L-tyrosine + tRNATyr(G34C) | the enzyme strictly recognizes the C1-G72 base pair, whereas the enzyme from Thermus thermophilus recognizes the G1-C72 in a different manner using different residues | Methanocaldococcus jannaschii | AMP + diphosphate + L-tyrosyl-tRNATyr(G34C) | - |
? | |
ATP + L-tyrosine + tRNATyr(G34C) | the enzyme strictly recognizes the C1-G72 base pair, whereas the enzyme from Thermus thermophilus recognizes the G1-C72 in a different manner using different residues | Methanocaldococcus jannaschii DSM 2661 | AMP + diphosphate + L-tyrosyl-tRNATyr(G34C) | - |
? | |
ATP + L-tyrosine + tRNATyr(wild-type) | the enzyme strictly recognizes the C1-G72 base pair, whereas the enzyme from Thermus thermophilus recognizes the G1-C72 in a different manner using different residues | Methanocaldococcus jannaschii | AMP + diphosphate + L-tyrosyl-tRNATyr(wild-type) | - |
? | |
ATP + L-tyrosine + tRNATyr(wild-type) | the enzyme strictly recognizes the C1-G72 base pair, whereas the enzyme from Thermus thermophilus recognizes the G1-C72 in a different manner using different residues | Methanocaldococcus jannaschii DSM 2661 | AMP + diphosphate + L-tyrosyl-tRNATyr(wild-type) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TyrRS | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
tRNATyr(G34C) | 37°C, pH 7.5, wild-type enzyme | Methanocaldococcus jannaschii | |
0.079 | - |
tRNATyr(G34C) | 37°C, pH 7.5, mutant enzyme D268R | Methanocaldococcus jannaschii | |
0.12 | - |
tRNATyr(wild-type) | 37°C, pH 7.5, mutant enzyme D268R | Methanocaldococcus jannaschii | |
0.19 | - |
tRNATyr(wild-type) | 37°C, pH 7.5, wild-type enzyme | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Methanocaldococcus jannaschii |