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Literature summary for 6.1.1.1 extracted from
- Engineering of an orthogonal aminoacyl-tRNA synthetase for efficient incorporation of the non-natural amino acid O-methyl-L-tyrosine using fluorescence-based bacterial cell sorting (2010), J. Mol. Biol., 404, 70-87.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | development of a one-plasmid expression system encoding an inducible modified tyrosyl-tRNA synthetase, the orthogonal cognate suppressor tRNA, and eGFPUAG in an individually regulatable fashion, overview. Assessment of the system for the incorporation of a non-natural amino acid yielding a fluorescent readout. Mutant library construction by random mutagenesis, and selection for O-methyl-L-tyrosine-specific mutant variants | Methanocaldococcus jannaschii |
| Y32Q/D158A/L162P/D286R | The engineered enzyme is improved with specificity for O-methyl-L-tyrosine and 10fold improved incorporation. The optimized synthetase is used for the preparative expression of a modified uvGFP carrying MeTyr at position 66 as part of its fluorophore. This biosynthetic protein shows quantitative incorporation of the non-natural amino acid, The Asp286Arg mutation serves for improved recognition of the CUA anticodon | Methanocaldococcus jannaschii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Tyrosyl-tRNA synthetase | - |
Methanocaldococcus jannaschii |
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