Cloned (Comment) | Organism |
---|---|
a library of more than 200 mutants substituting the ATP binding motif KMSSS, Lys204-Met205-Ser206-Ser207-Ser208, is built, mutants and wild-type of MjYRS are cloned into the vector pET41a+ | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
AMSSS | TyrRS mutant, a library of more than 200 mutants substituting the ATP binding motif KMSSS is built | Methanocaldococcus jannaschii |
KMGCA | TyrRS mutant, a library of more than 200 mutants substituting the ATP binding motif KMSSS is built | Methanocaldococcus jannaschii |
RMSSS | TyrRS mutant, a library of more than 200 mutants substituting the ATP binding motif KMSSS is built | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
L-tyrosine | mutant TyrRS, KMGCA | Methanocaldococcus jannaschii | |
0.0043 | - |
L-tyrosine | mutant TyrRS, RMSSS | Methanocaldococcus jannaschii | |
0.0043 | - |
L-tyrosine | wild-type TyrRS, KMSSS | Methanocaldococcus jannaschii | |
0.0044 | - |
L-tyrosine | mutant TyrRS, AMSSS | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + tRNATyr | Methanocaldococcus jannaschii | - |
AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by chromatography on Ni-nitrilotriacetic acid agarose | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + tRNATyr | - |
Methanocaldococcus jannaschii | AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MjYRS | - |
Methanocaldococcus jannaschii |
Tyrosyl-tRNA synthetase | - |
Methanocaldococcus jannaschii |
TyrRS | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
tyrosylation assay | Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
L-tyrosine | mutant TyrRS, RMSSS | Methanocaldococcus jannaschii | |
0.012 | - |
L-tyrosine | mutant TyrRS, AMSSS | Methanocaldococcus jannaschii | |
0.025 | - |
L-tyrosine | mutant TyrRS, KMGCA | Methanocaldococcus jannaschii | |
0.53 | - |
L-tyrosine | wild-type TyrRS, KMSSS | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
tyrosylation assay | Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methanocaldococcus jannaschii |