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Literature summary for 6.1.1.1 extracted from

  • Kamijo, S.; Fujii, A.; Onodera, K.; Wakabayashi, K.
    Analyses of conditions for KMSSS loop in tyrosyl-tRNA synthetase by building a mutant library (2009), J. Biochem., 146, 241-250.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
a library of more than 200 mutants substituting the ATP binding motif KMSSS, Lys204-Met205-Ser206-Ser207-Ser208, is built, mutants and wild-type of MjYRS are cloned into the vector pET41a+ Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
AMSSS TyrRS mutant, a library of more than 200 mutants substituting the ATP binding motif KMSSS is built Methanocaldococcus jannaschii
KMGCA TyrRS mutant, a library of more than 200 mutants substituting the ATP binding motif KMSSS is built Methanocaldococcus jannaschii
RMSSS TyrRS mutant, a library of more than 200 mutants substituting the ATP binding motif KMSSS is built Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
L-tyrosine mutant TyrRS, KMGCA Methanocaldococcus jannaschii
0.0043
-
L-tyrosine mutant TyrRS, RMSSS Methanocaldococcus jannaschii
0.0043
-
L-tyrosine wild-type TyrRS, KMSSS Methanocaldococcus jannaschii
0.0044
-
L-tyrosine mutant TyrRS, AMSSS Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tyrosine + tRNATyr Methanocaldococcus jannaschii
-
AMP + diphosphate + L-tyrosyl-tRNATyr
-
?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by chromatography on Ni-nitrilotriacetic acid agarose Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tyrosine + tRNATyr
-
Methanocaldococcus jannaschii AMP + diphosphate + L-tyrosyl-tRNATyr
-
?

Synonyms

Synonyms Comment Organism
MjYRS
-
Methanocaldococcus jannaschii
Tyrosyl-tRNA synthetase
-
Methanocaldococcus jannaschii
TyrRS
-
Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
tyrosylation assay Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
L-tyrosine mutant TyrRS, RMSSS Methanocaldococcus jannaschii
0.012
-
L-tyrosine mutant TyrRS, AMSSS Methanocaldococcus jannaschii
0.025
-
L-tyrosine mutant TyrRS, KMGCA Methanocaldococcus jannaschii
0.53
-
L-tyrosine wild-type TyrRS, KMSSS Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
tyrosylation assay Methanocaldococcus jannaschii

Cofactor

Cofactor Comment Organism Structure
ATP
-
Methanocaldococcus jannaschii