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Literature summary for 6.1.1.1 extracted from

  • Abergel, C.; Rudinger-Thirion, J.; Giege, R.; Claverie, J.M.
    Virus-encoded aminoacyl-tRNA synthetases: structural and functional characterization of mimivirus TyrRS and MetRS (2007), J. Virol., 81, 12406-12417.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
TyrRS, phylogenetic analysis, the viral aminoacyl tRNA synthetases have not been acquired recently by horizontal gene transfer from a cellular host but rather militate in favor of an intricate evolutionary relationship between large DNA viruses and ancestral eukaryotes Acanthamoeba polyphaga Mimivirus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified selenomethionyl-TyrRSapm in complex with tyrosinol, crystallization is improved by introducing anion-exchange chromatography, vapour diffusion method, 0.002 ml of 14 mg/ml protein in 20 mM Tris-HCl, pH 7.4, 25°C, is mixed with 500 nl reservoir solution containing 0.1 M sodium citrate, pH 5.5, and 6-9% PEG 4000 w/v, 15% 2-methyl-2,4-pentane-d12-diol, 0.1 M KCl, 1 mM MgCl2, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular replacement Acanthamoeba polyphaga Mimivirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics with Escherichia coli tRNATyr, Plasmodium falciparum tRNATyr, and diverse wild-type and mutant Saccharomyces cerevisiae tRNATyrs, overview Acanthamoeba polyphaga Mimivirus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Acanthamoeba polyphaga Mimivirus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tyrosine + tRNATyr Acanthamoeba polyphaga Mimivirus the TyrRS specificity for tyrosine and conformity with the identity rules for tRNATyr for archea/eukarya, anticodon binding site, overview AMP + diphosphate + L-tyrosyl-tRNATyr
-
?
additional information Acanthamoeba polyphaga Mimivirus mimivirus aminoacyl-tRNA synthetases function as regular translation enzymes in infected amoebas ?
-
?

Organism

Organism UniProt Comment Textmining
Acanthamoeba polyphaga Mimivirus Q5UPJ7
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity with Escherichia coli tRNATyr, Plasmodium falciparum tRNATyr, and diverse wild-type and mutant Saccharomyces cerevisiae tRNATyrs, overview Acanthamoeba polyphaga Mimivirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tyrosine + tRNATyr the TyrRS specificity for tyrosine and conformity with the identity rules for tRNATyr for archea/eukarya, anticodon binding site, overview Acanthamoeba polyphaga Mimivirus AMP + diphosphate + L-tyrosyl-tRNATyr
-
?
ATP + L-tyrosine + tRNATyr functional idiosyncrasies of the viral TyrRS, activity with Escherichia coli tRNATyr, Plasmodium falciparum tRNATyr, and diverse wild-type and mutant Saccharomyces cerevisiae tRNATyrs, overview, the TyrRS specificity for tyrosine and recognition of the tRNATyr acceptor stem show conformity with the identity rules for tRNATyr for archea/eukarya, anticodon binding site, overview Acanthamoeba polyphaga Mimivirus AMP + diphosphate + L-tyrosyl-tRNATyr
-
?
additional information mimivirus aminoacyl-tRNA synthetases function as regular translation enzymes in infected amoebas Acanthamoeba polyphaga Mimivirus ?
-
?

Subunits

Subunits Comment Organism
dimer dimer interface structure from crystal structure analysis, modeling, overview Acanthamoeba polyphaga Mimivirus

Synonyms

Synonyms Comment Organism
Tyrosyl-tRNA synthetase
-
Acanthamoeba polyphaga Mimivirus
TyrRS
-
Acanthamoeba polyphaga Mimivirus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Acanthamoeba polyphaga Mimivirus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Acanthamoeba polyphaga Mimivirus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Acanthamoeba polyphaga Mimivirus