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Literature summary for 6.1.1.1 extracted from

  • Jia, J.; Li, B.; Jin, Y.; Wang, D.
    Expression, purification, and characterization of human tyrosyl-tRNA synthetase (2003), Protein Expr. Purif., 27, 104-108.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene TyrS, DNA sequence determination, overexpression as His-tagged enzyme in Escherichia coli BL21 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information the C-terminal His-tag of the recombinant enzyme has little effect on the catalytic activity Homo sapiens
0.0003
-
L-tyrosine 30°C, purified recombinant His-tagged enzyme Homo sapiens
0.0009
-
tRNATyr 30°C, purified recombinant His-tagged enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
2 * 58000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tyrosine + tRNATyr Homo sapiens
-
AMP + L-Tyr-tRNATyr + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
gene TyrS
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21, 4.2fold Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
95D cell lung cancer giant-cell strain Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000076
-
purified recombinant His-tagged enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tyrosine + tRNATyr
-
Homo sapiens AMP + L-Tyr-tRNATyr + diphosphate
-
?
ATP + L-tyrosine + tRNATyr 2-step reaction, 1. activation of L-tyrosine with ATP to form L-Tyr-AMP, 2. transfer of the tyrosyl-group to tRNATyr Homo sapiens AMP + L-Tyr-tRNATyr + diphosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 58000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
Tyrosyl-tRNA synthetase
-
Homo sapiens
TyrRS
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information the C-terminal His-tag of the recombinant enzyme has little effect on the catalytic activity Homo sapiens
1.49
-
L-tyrosine 30°C, purified recombinant His-tagged enzyme Homo sapiens
6.08
-
L-tyrosine 30°C, purified recombinant His-tagged enzyme Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens