Cloned (Comment) | Organism |
---|---|
gene TyrS, DNA sequence determination, overexpression as His-tagged enzyme in Escherichia coli BL21 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the C-terminal His-tag of the recombinant enzyme has little effect on the catalytic activity | Homo sapiens | |
0.0003 | - |
L-tyrosine | 30°C, purified recombinant His-tagged enzyme | Homo sapiens | |
0.0009 | - |
tRNATyr | 30°C, purified recombinant His-tagged enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
2 * 58000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + tRNATyr | Homo sapiens | - |
AMP + L-Tyr-tRNATyr + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
gene TyrS | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21, 4.2fold | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
95D cell | lung cancer giant-cell strain | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000076 | - |
purified recombinant His-tagged enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + tRNATyr | - |
Homo sapiens | AMP + L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + L-tyrosine + tRNATyr | 2-step reaction, 1. activation of L-tyrosine with ATP to form L-Tyr-AMP, 2. transfer of the tyrosyl-group to tRNATyr | Homo sapiens | AMP + L-Tyr-tRNATyr + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Tyrosyl-tRNA synthetase | - |
Homo sapiens |
TyrRS | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the C-terminal His-tag of the recombinant enzyme has little effect on the catalytic activity | Homo sapiens | |
1.49 | - |
L-tyrosine | 30°C, purified recombinant His-tagged enzyme | Homo sapiens | |
6.08 | - |
L-tyrosine | 30°C, purified recombinant His-tagged enzyme | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |