Application | Comment | Organism |
---|---|---|
biotechnology | use of mutant Y43G for specialized protein synthesis as a carrier for additional amino acids and derivatives for use in e.g. crystal structure determination by X-ray diffraction | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
overexpression of His-tagged wild-type and mutant Y43G enzyme in Escherichia coli BL21(DE3) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
Y43G | broadened amino acid substrate specificity | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
L-tyrosine | pH 7.6, 30°C, wild-type enzyme, radioisotopic assay | Saccharomyces cerevisiae | |
0.014 | - |
L-tyrosine | pH 7.6, 30°C, wild-type enzyme, spectrophotometric assay | Saccharomyces cerevisiae | |
0.46 | - |
D-tyrosine | pH 7.6, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
0.56 | - |
L-3,4-dihydroxyphenylalanine | pH 7.6, 30°C, mutant Y43G | Saccharomyces cerevisiae | |
0.625 | - |
3-fluoro-D,L-tyrosine | pH 7.6, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
1.15 | - |
3-iodo-L-tyrosine | pH 7.6, 30°C, mutant Y43G | Saccharomyces cerevisiae | |
1.19 | - |
L-tyrosine | pH 7.6, 30°C, mutant Y43G | Saccharomyces cerevisiae | |
1.3 | - |
3-fluoro-D,L-tyrosine | pH 7.6, 30°C, mutant Y43G | Saccharomyces cerevisiae | |
1.84 | - |
L-3,4-dihydroxyphenylalanine | pH 7.6, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
2.04 | - |
3-chloro-L-tyrosine | pH 7.6, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
2.43 | - |
3-chloro-L-tyrosine | pH 7.6, 30°C, mutant Y43G | Saccharomyces cerevisiae | |
14 | - |
D-tyrosine | pH 7.6, 30°C, mutant Y43G | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + tRNATyr | Saccharomyces cerevisiae | - |
AMP + L-Tyr-tRNATyr + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant Y43G enzyme from Escherichia coli Y43G | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-amino-L-tyrosine + tRNATyr | mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + 3-amino-L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + 3-azido-L-tyrosine + tRNATyr | mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + 3-azido-L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + 3-chloro-L-tyrosine + tRNATyr | mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + 3-chloro-L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + 3-iodo-L-tyrosine + tRNATyr | mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + 3-iodo-L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + 3-methoxy-L-tyrosine + tRNATyr | mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + 3-methoxy-L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + 3-nitro-L-tyrosine + tRNATyr | mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + 3-nitro-L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + D-3,4-dihydroxyphenylalanine + tRNATyr | mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + D-3,4-dihydroxyphenylalanine-tRNATyr + diphosphate | - |
? | |
ATP + D-tyrosine + tRNATyr | mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + D-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + L-3,4-dihydroxyphenylalanine + tRNATyr | mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + L-3,4-dihydroxyphenylalanine-tRNATyr + diphosphate | - |
? | |
ATP + L-tyrosine + tRNATyr | - |
Saccharomyces cerevisiae | AMP + L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + L-tyrosine + tRNATyr | wild-type and mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + m-fluoro-D,L-tyrosine + tRNATyr | mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + m-fluoro-D,L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + N-acetyl-L-tyrosine + tRNATyr | wild-type and mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + N-acetyl-L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + N-formyl-L-tyrosine + tRNATyr | wild-type and mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + N-formyl-L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + N-o-nitrophenylsulfenyl-L-tyrosine + tRNATyr | wild-type and mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + N-o-nitrophenylsulfenyl-L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + O-dansyl-L-tyrosine + tRNATyr | wild-type and mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + O-dansyl-L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + O-methyl-L-tyrosine + tRNATyr | wild-type and mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + O-methyl-L-Tyr-tRNATyr + diphosphate | - |
? | |
ATP + O-phospho-L-tyrosine + tRNATyr | wild-type and mutant Y43G, aminoacylation assay | Saccharomyces cerevisiae | AMP + O-phospho-L-Tyr-tRNATyr + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tyrosine tRNA synthetase | - |
Saccharomyces cerevisiae |
TyrRS | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
3-chloro-L-tyrosine | pH 7.6, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
0.37 | - |
D-tyrosine | pH 7.6, 30°C, mutant Y43G | Saccharomyces cerevisiae | |
0.48 | - |
3-iodo-L-tyrosine | pH 7.6, 30°C, mutant Y43G | Saccharomyces cerevisiae | |
0.62 | - |
3-fluoro-D,L-tyrosine | pH 7.6, 30°C, mutant Y43G | Saccharomyces cerevisiae | |
0.67 | - |
L-3,4-dihydroxyphenylalanine | pH 7.6, 30°C, mutant Y43G | Saccharomyces cerevisiae | |
0.95 | - |
L-tyrosine | pH 7.6, 30°C, mutant Y43G | Saccharomyces cerevisiae | |
1.2 | - |
D-tyrosine | pH 7.6, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
1.46 | - |
3-chloro-L-tyrosine | pH 7.6, 30°C, mutant Y43G | Saccharomyces cerevisiae | |
1.65 | - |
L-3,4-dihydroxyphenylalanine | pH 7.6, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
3.3 | - |
3-fluoro-D,L-tyrosine | pH 7.6, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
4.4 | - |
L-tyrosine | pH 7.6, 30°C, wild-type enzyme, radioisotopic assay | Saccharomyces cerevisiae | |
4.6 | - |
L-tyrosine | pH 7.6, 30°C, wild-type enzyme, spectrophotometric assay | Saccharomyces cerevisiae | |
4.96 | - |
3-fluoro-D,L-tyrosine | pH 7.6, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
6.08 | - |
L-3,4-dihydroxyphenylalanine | pH 7.6, 30°C, mutant Y43G | Saccharomyces cerevisiae | |
6.08 | - |
3-fluoro-D,L-tyrosine | pH 7.6, 30°C, mutant Y43G | Saccharomyces cerevisiae | |
6.08 | - |
3-chloro-L-tyrosine | pH 7.6, 30°C, mutant Y43G | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |