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Literature summary for 6.1.1.1 extracted from

  • Ohno, S.; Yokogawa, T.; Nishikawa, K.
    Changing the amino acid specificity of yeast tyrosyl-tRNA synthetase by genetic engineering (2001), J. Biochem., 130, 417-423.
    View publication on PubMed

Application

Application Comment Organism
biotechnology use of mutant Y43G for specialized protein synthesis as a carrier for additional amino acids and derivatives for use in e.g. crystal structure determination by X-ray diffraction Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged wild-type and mutant Y43G enzyme in Escherichia coli BL21(DE3) Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
Y43G broadened amino acid substrate specificity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
L-tyrosine pH 7.6, 30°C, wild-type enzyme, radioisotopic assay Saccharomyces cerevisiae
0.014
-
L-tyrosine pH 7.6, 30°C, wild-type enzyme, spectrophotometric assay Saccharomyces cerevisiae
0.46
-
D-tyrosine pH 7.6, 30°C, wild-type enzyme Saccharomyces cerevisiae
0.56
-
L-3,4-dihydroxyphenylalanine pH 7.6, 30°C, mutant Y43G Saccharomyces cerevisiae
0.625
-
3-fluoro-D,L-tyrosine pH 7.6, 30°C, wild-type enzyme Saccharomyces cerevisiae
1.15
-
3-iodo-L-tyrosine pH 7.6, 30°C, mutant Y43G Saccharomyces cerevisiae
1.19
-
L-tyrosine pH 7.6, 30°C, mutant Y43G Saccharomyces cerevisiae
1.3
-
3-fluoro-D,L-tyrosine pH 7.6, 30°C, mutant Y43G Saccharomyces cerevisiae
1.84
-
L-3,4-dihydroxyphenylalanine pH 7.6, 30°C, wild-type enzyme Saccharomyces cerevisiae
2.04
-
3-chloro-L-tyrosine pH 7.6, 30°C, wild-type enzyme Saccharomyces cerevisiae
2.43
-
3-chloro-L-tyrosine pH 7.6, 30°C, mutant Y43G Saccharomyces cerevisiae
14
-
D-tyrosine pH 7.6, 30°C, mutant Y43G Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tyrosine + tRNATyr Saccharomyces cerevisiae
-
AMP + L-Tyr-tRNATyr + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant Y43G enzyme from Escherichia coli Y43G Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-amino-L-tyrosine + tRNATyr mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + 3-amino-L-Tyr-tRNATyr + diphosphate
-
?
ATP + 3-azido-L-tyrosine + tRNATyr mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + 3-azido-L-Tyr-tRNATyr + diphosphate
-
?
ATP + 3-chloro-L-tyrosine + tRNATyr mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + 3-chloro-L-Tyr-tRNATyr + diphosphate
-
?
ATP + 3-iodo-L-tyrosine + tRNATyr mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + 3-iodo-L-Tyr-tRNATyr + diphosphate
-
?
ATP + 3-methoxy-L-tyrosine + tRNATyr mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + 3-methoxy-L-Tyr-tRNATyr + diphosphate
-
?
ATP + 3-nitro-L-tyrosine + tRNATyr mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + 3-nitro-L-Tyr-tRNATyr + diphosphate
-
?
ATP + D-3,4-dihydroxyphenylalanine + tRNATyr mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + D-3,4-dihydroxyphenylalanine-tRNATyr + diphosphate
-
?
ATP + D-tyrosine + tRNATyr mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + D-Tyr-tRNATyr + diphosphate
-
?
ATP + L-3,4-dihydroxyphenylalanine + tRNATyr mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + L-3,4-dihydroxyphenylalanine-tRNATyr + diphosphate
-
?
ATP + L-tyrosine + tRNATyr
-
Saccharomyces cerevisiae AMP + L-Tyr-tRNATyr + diphosphate
-
?
ATP + L-tyrosine + tRNATyr wild-type and mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + L-Tyr-tRNATyr + diphosphate
-
?
ATP + m-fluoro-D,L-tyrosine + tRNATyr mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + m-fluoro-D,L-Tyr-tRNATyr + diphosphate
-
?
ATP + N-acetyl-L-tyrosine + tRNATyr wild-type and mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + N-acetyl-L-Tyr-tRNATyr + diphosphate
-
?
ATP + N-formyl-L-tyrosine + tRNATyr wild-type and mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + N-formyl-L-Tyr-tRNATyr + diphosphate
-
?
ATP + N-o-nitrophenylsulfenyl-L-tyrosine + tRNATyr wild-type and mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + N-o-nitrophenylsulfenyl-L-Tyr-tRNATyr + diphosphate
-
?
ATP + O-dansyl-L-tyrosine + tRNATyr wild-type and mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + O-dansyl-L-Tyr-tRNATyr + diphosphate
-
?
ATP + O-methyl-L-tyrosine + tRNATyr wild-type and mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + O-methyl-L-Tyr-tRNATyr + diphosphate
-
?
ATP + O-phospho-L-tyrosine + tRNATyr wild-type and mutant Y43G, aminoacylation assay Saccharomyces cerevisiae AMP + O-phospho-L-Tyr-tRNATyr + diphosphate
-
?

Synonyms

Synonyms Comment Organism
Tyrosine tRNA synthetase
-
Saccharomyces cerevisiae
TyrRS
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
3-chloro-L-tyrosine pH 7.6, 30°C, wild-type enzyme Saccharomyces cerevisiae
0.37
-
D-tyrosine pH 7.6, 30°C, mutant Y43G Saccharomyces cerevisiae
0.48
-
3-iodo-L-tyrosine pH 7.6, 30°C, mutant Y43G Saccharomyces cerevisiae
0.62
-
3-fluoro-D,L-tyrosine pH 7.6, 30°C, mutant Y43G Saccharomyces cerevisiae
0.67
-
L-3,4-dihydroxyphenylalanine pH 7.6, 30°C, mutant Y43G Saccharomyces cerevisiae
0.95
-
L-tyrosine pH 7.6, 30°C, mutant Y43G Saccharomyces cerevisiae
1.2
-
D-tyrosine pH 7.6, 30°C, wild-type enzyme Saccharomyces cerevisiae
1.46
-
3-chloro-L-tyrosine pH 7.6, 30°C, mutant Y43G Saccharomyces cerevisiae
1.65
-
L-3,4-dihydroxyphenylalanine pH 7.6, 30°C, wild-type enzyme Saccharomyces cerevisiae
3.3
-
3-fluoro-D,L-tyrosine pH 7.6, 30°C, wild-type enzyme Saccharomyces cerevisiae
4.4
-
L-tyrosine pH 7.6, 30°C, wild-type enzyme, radioisotopic assay Saccharomyces cerevisiae
4.6
-
L-tyrosine pH 7.6, 30°C, wild-type enzyme, spectrophotometric assay Saccharomyces cerevisiae
4.96
-
3-fluoro-D,L-tyrosine pH 7.6, 30°C, wild-type enzyme Saccharomyces cerevisiae
6.08
-
L-3,4-dihydroxyphenylalanine pH 7.6, 30°C, mutant Y43G Saccharomyces cerevisiae
6.08
-
3-fluoro-D,L-tyrosine pH 7.6, 30°C, mutant Y43G Saccharomyces cerevisiae
6.08
-
3-chloro-L-tyrosine pH 7.6, 30°C, mutant Y43G Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae