Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of diverse tRNA variants of the native tRNATyr from yeast by heterologous in vitro translation, transplantation and point mutations | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, influence of assay conditions, Km for diverse tRNA variants, overview | Saccharomyces cerevisiae | |
0.002 | - |
native yeast tRNATyr | pH 7.5, 30°C | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + tyrosine + tRNATyr | Saccharomyces cerevisiae | - |
AMP + Tyr-tRNATyr + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + native yeast tRNATyr | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? | |
ATP + tyrosine + tRNATyr | - |
Saccharomyces cerevisiae | AMP + Tyr-tRNATyr + diphosphate | - |
? | |
ATP + tyrosine + tRNATyr | heterologous tRNATyr from Escherichia coli and Methanococcus jannashii, tyrosylation efficiency of tRNA variants: determinants are base pair C1-G72, discriminator residue A73, and the 3 anticodon bases G34, U35, and A36 | Saccharomyces cerevisiae | AMP + Tyr-tRNATyr + diphosphate | - |
? | |
additional information | no activity with tRNAAsp and tRNAPhe | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tyrosyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
TyrRS | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | influence of assay conditions, kcat for diverse tRNA variants, overview | Saccharomyces cerevisiae | |
1.5 | - |
native yeast tRNATyr | pH 7.5, 30°C | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |