Protein Variants | Comment | Organism |
---|---|---|
additional information | production of enzyme derivatives in which the Cys residue has been converted to a series of unnatural amino acids. The results demonstrate that the Cys residue does not function as an acid-base or nucleophilic group during catalysis nor does it play any role in the substrate inhibition | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4,2',4'-trihydroxychalcone | substrate inhibition | Glycine max | |
4,4'-Dihydroxychalcone | - |
Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
4,2',4',6'-Tetrahydroxychalcone | methanethiolated enzyme | Glycine max | |
0.0063 | - |
4,2',4',6'-Tetrahydroxychalcone | native enzyme | Glycine max | |
0.12 | - |
4,2',4'-trihydroxychalcone | methanethiolated enzyme | Glycine max | |
0.18 | - |
4,2',4'-trihydroxychalcone | native enzyme | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-Hydroxy-4-methoxychalcone | - |
Glycine max | ? | - |
? | |
4,2',4',6'-Tetrahydroxychalcone | - |
Glycine max | 5,7,4'-Trihydroxyflavanone | - |
? | |
4,2',4'-trihydroxychalcone | - |
Glycine max | (2S)-4',7-dihydroxyflavanone | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
107 | - |
4,2',4'-trihydroxychalcone | methanethiolated enzyme | Glycine max | |
167 | - |
4,2',4'-trihydroxychalcone | native enzyme | Glycine max | |
700 | - |
4,2',4',6'-Tetrahydroxychalcone | methanethiolated enzyme | Glycine max | |
833 | - |
4,2',4',6'-Tetrahydroxychalcone | native enzyme | Glycine max |