Inhibitors | Comment | Organism | Structure |
---|---|---|---|
27,28-bisnoroxidosqualene | irreversible inhibition | Sus scrofa | |
27-norsqualene | - |
Sus scrofa | |
28-noroxidosqualene | - |
Sus scrofa | |
29-norsqualene | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
(3S)-2,3-epoxy-2,3-dihydrosqualene | pH 7.4, 37°C, liver microsomes | Sus scrofa | |
0.231 | - |
3-[(3E,7E,15E)-16-ethyl-3,7,12,20-tetramethylhenicosa-3,7,11,15,19-pentaen-1-yl]-2,2-dimethyloxirane | pH 7.4, 37°C, liver microsomes | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Sus scrofa | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene | Sus scrofa | - |
lanosterol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
liver microsome preparation | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Sus scrofa | lanosterol | - |
? | |
(3S)-2,3-epoxy-2,3-dihydrosqualene | the enzyme performs the polycyclization reaction by folding the substrate in a chair-boat-chair-chair conformation | Sus scrofa | lanosterol | - |
? | |
27-norsqualene | - |
Sus scrofa | 30-norlanosterol + 26-normalabaricatriene | - |
? | |
29-norsqualene | - |
Sus scrofa | (2S,4aS,6aR,7R,10aR,12aR)-1,1,4a,6a,10a-pentamethyl-7-(4-methylpent-3-en-1-yl)-1,2,3,4,4a,5,6,6a,7,8,9,10,10a,11,12,12a-hexadecahydrochrysen-2-ol + (2S,4aS,6aS,7R,10aS,12aR)-1,1,4a,6a,10a-pentamethyl-7-(4-methylpent-3-en-1-yl)-1,2,3,4,4a,5,6,6a,7,8,9,10,10a,11,12,12a-hexadecahydrochrysen-2-ol | - |
? | |
3-[(3E,7E,15E)-16-ethyl-3,7,12,20-tetramethylhenicosa-3,7,11,15,19-pentaen-1-yl]-2,2-dimethyloxirane | ethylation at C10 or C15 | Sus scrofa | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000013 | - |
27-norsqualene | pH 7.4, 37°C, liver microsomes | Sus scrofa | |
0.000015 | - |
29-norsqualene | pH 7.4, 37°C, liver microsomes | Sus scrofa | |
0.00002 | - |
28-noroxidosqualene | pH 7.4, 37°C, liver microsomes | Sus scrofa |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.076 | - |
pH 7.4, 37°C, liver microsomes | Sus scrofa | 29-norsqualene | |
0.18 | - |
pH 7.4, 37°C, liver microsomes | Sus scrofa | 27-norsqualene |