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Literature summary for 5.4.99.17 extracted from
- Kinetic studies on the function of all the conserved tryptophans involved inside and outside the QW motifs of squalene-hopene cyclase: stabilizing effect of the protein structure against thermal denaturation (1999), Biosci. Biotechnol. Biochem., 63, 1171-1180.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Alicyclobacillus acidocaldarius |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| W23V | same activity and optimal temperature as wild type enzyme | Alicyclobacillus acidocaldarius |
| W258L | 60% of wild type activity, lower temperature optimum | Alicyclobacillus acidocaldarius |
| W406V | no enzyme activity | Alicyclobacillus acidocaldarius |
| W417A | no enzyme activity | Alicyclobacillus acidocaldarius |
| W485V | same activity and optimal temperature as wild type enzyme | Alicyclobacillus acidocaldarius |
| W522V | same activity and optimal temperature as wild type enzyme | Alicyclobacillus acidocaldarius |
| W533A | same activity and optimal temperature as wild type enzyme | Alicyclobacillus acidocaldarius |
| W591L | same activity and optimal temperature as wild type enzyme | Alicyclobacillus acidocaldarius |
| W78S | same activity and optimal temperature as wild type enzyme | Alicyclobacillus acidocaldarius |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km for wild type and mutant enzymes | Alicyclobacillus acidocaldarius | |
| 0.0167 | - |
squalene | - |
Alicyclobacillus acidocaldarius |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alicyclobacillus acidocaldarius | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Alicyclobacillus acidocaldarius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activities for wild type and mutant enzymes | Alicyclobacillus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| squalene | - |
Alicyclobacillus acidocaldarius | hop-22(29)-ene | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
wild type and mutant enzymes | Alicyclobacillus acidocaldarius |
| 55 | - |
W258L mutant | Alicyclobacillus acidocaldarius |
| 60 | - |
- |
Alicyclobacillus acidocaldarius |
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Release 2023.1 (January 2023)
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