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Literature summary for 5.4.99.17 extracted from

  • Ochs, D.; Tappe, C.H.; Gaertner, P.; Kellner, R.; Poralla, K.
    Properties of purified squalene-hopene cyclase from Bacillus acidocaldarius (1990), Eur. J. Biochem., 194, 75-80.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
sodium taurodeoxycholate 1.5fold activation at 0.16% Alicyclobacillus acidocaldarius

Inhibitors

Inhibitors Comment Organism Structure
(5-hydroxycarvacryl)trimethylammonium chloride 1-piperidine carboxylate i.e. AMO 1618, competitive inhibition Alicyclobacillus acidocaldarius
azasqualene inhibition at 0.001 mM Alicyclobacillus acidocaldarius
Cu2+ slight inhibition at 1 mM Alicyclobacillus acidocaldarius
dodecyldimethylamine N-oxide competitive inhibition Alicyclobacillus acidocaldarius
dodecyltrimethylammonium bromide competitive inhibition, 50% inhibition at 0.0001 mM Alicyclobacillus acidocaldarius
farnesol inhibition at 0.1 mM Alicyclobacillus acidocaldarius
Fe2+ slight inhibition at 1 mM Alicyclobacillus acidocaldarius
additional information inhibition by n-alkyldimethylammoniumhalides with alkyl chain lengths between 12 and 18 C atoms, inhibition increases with decreasing chain length Alicyclobacillus acidocaldarius
sodium dodecylsulfate strong inhibition Alicyclobacillus acidocaldarius
sodium taurodeoxycholate under 0.15% and above 0.25% Alicyclobacillus acidocaldarius
Zn2+ slight inhibition at 5 mM Alicyclobacillus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
squalene
-
Alicyclobacillus acidocaldarius

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane
-
Alicyclobacillus acidocaldarius
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
2 * 75000, SDS-PAGE, enzyme forms aggregates in absence of detergent Alicyclobacillus acidocaldarius
150000
-
gel filtration Alicyclobacillus acidocaldarius

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Alicyclobacillus acidocaldarius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.345
-
-
Alicyclobacillus acidocaldarius

Subunits

Subunits Comment Organism
dimer 2 * 75000, SDS-PAGE, enzyme forms aggregates in absence of detergent Alicyclobacillus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Alicyclobacillus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Alicyclobacillus acidocaldarius

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00014
-
dodecyldimethylamine N-oxide
-
Alicyclobacillus acidocaldarius
0.00032
-
dodecyltrimethylammonium bromide
-
Alicyclobacillus acidocaldarius
0.00054
-
(5-hydroxycarvacryl)trimethylammonium chloride 1-piperidine carboxylate
-
Alicyclobacillus acidocaldarius