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Literature summary for 5.4.99.17 extracted from

  • Feil, C.; Suessmuth, R.; Jung, G.; Poralla, K.
    Site-directed mutagenesis of putative active-site residues in squalene-hopene cyclase (1996), Eur. J. Biochem., 242, 51-55.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
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Alicyclobacillus acidocaldarius

Protein Variants

Protein Variants Comment Organism
D376E 10% enzyme activity Alicyclobacillus acidocaldarius
D376E/D377E no enzyme activity Alicyclobacillus acidocaldarius
D376G 0.2% activity when enzyme concentration is increased to 100fold Alicyclobacillus acidocaldarius
D376Q no enzyme activity Alicyclobacillus acidocaldarius
D376R no enzyme activity Alicyclobacillus acidocaldarius
D377E 0.2% activity when enzyme concentration is increased to 100fold Alicyclobacillus acidocaldarius
D377G 0.2% activity when enzyme concentration is increased to 100fold Alicyclobacillus acidocaldarius
D377Q 0.2% activity when enzyme concentration is increased to 100fold Alicyclobacillus acidocaldarius
D377R no enzyme activity Alicyclobacillus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
squalene wild type enzyme and D376E mutant Alicyclobacillus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
SDS-PAGE Alicyclobacillus acidocaldarius

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius
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-
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Purification (Commentary)

Purification (Comment) Organism
wild type and mutants, homogeneity Alicyclobacillus acidocaldarius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activities for wild type and mutant enzymes Alicyclobacillus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
wild type enzyme: 50% loss of activity after 120 min, D376E mutant: 50% loss of activity after 100 min Alicyclobacillus acidocaldarius