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Literature summary for 5.4.99.12 extracted from

  • Zhao, X.; Horne, D.A.
    The role of cysteine residues in the rearrangement of uridine to pseudouridine catalyzed by pseudouridine synthase I (1997), J. Biol. Chem., 272, 1950-1955.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
C154A activity similar to wild-type Escherichia coli
C154A mutant shows high levels of activity Escherichia coli
C154S no activity Escherichia coli
C154S complete loss of activity, mutant is capable of binding to substrate tRNAPhe Escherichia coli
C169A activity similar to wild-type Escherichia coli
C169A mutant shows high levels of activity Escherichia coli
C169S no activity Escherichia coli
C169S complete loss of activity, mutant is unable to bind substrate tRNAPhe Escherichia coli
C55A/C154A/C169A activity similar to wild-type Escherichia coli
C55A/C154A/C169A only a small change in both Km and Vmax parameters as compared with wild-type enzyme. These mutations cause a 1.2fold increase in Km and a 1.6fold decrease in Vmax. The overall Vmax/Km ratio is lowered by a factor of 2 for the triple mutant Escherichia coli
C55S activity similar to wild-type Escherichia coli
C55S maintains activity levels similar to those of wild-type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000086
-
Escherichia coli tRNAPhe uridine39 wild-type, pH 8.0, 30°C Escherichia coli
0.000086
-
tRNA uridine39 pH 8.0, 30°, wild-type enzyme Escherichia coli
0.00011
-
tRNA uridine39 pH 8.0, 30°C, mutant enzyme C55A/C154A/C169A Escherichia coli
0.00011
-
Escherichia coli tRNAPhe uridine39 mutant C55A/C154A/C169A, pH 8.0, 30°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
x * 31000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
tRNA uridine38-40 = tRNA pseudouridine38-40 chemical evidence against a covalent cysteine intermediate in the rearrangement mechanism of uridine to pseudouridine Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Escherichia coli tRNAPhe uridine39
-
Escherichia coli Escherichia coli tRNAPhe pseudouridine39
-
?
tRNA uridine39 tRNAPhe with uridine at position 39 Escherichia coli tRNA pseudouridine39
-
?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
PSUI
-
Escherichia coli
tRNA pseudouridine synthase I
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Escherichia coli