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Literature summary for 5.4.4.2 extracted from

  • Kolappan, S.; Zwahlen, J.; Zhou, R.; Truglio, J.J.; Tonge, P.J.; Kisker, C.
    Lysine 190 is the catalytic base in MenF, the menaquinone-specific isochorismate synthase from Escherichia coli: implications for an enzyme family (2007), Biochemistry, 46, 946-953.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
at 2.5 A resolution Escherichia coli

Protein Variants

Protein Variants Comment Organism
A344T no catalytic activity Escherichia coli
E240Q no catalytic activity Escherichia coli
K90A no catalytic activity Escherichia coli
L255A 50% increase in Km value, 90% decrease in kcat value Escherichia coli
R387A no catalytic activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.192
-
chorismate wild-type, pH 7.5, 37°C Escherichia coli
0.319
-
chorismate mutant L255A, pH 7.5, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P38051 menaquinone-specific enzyme
-

Reaction

Reaction Comment Organism Reaction ID
chorismate = isochorismate K90 is the base that activates water for nucleophilic attack at the chorismate C2 carbon Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate
-
Escherichia coli Isochorismate
-
?

Synonyms

Synonyms Comment Organism
MenF
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.031
-
chorismate mutant L255A, pH 7.5, 37°C Escherichia coli
0.35
-
chorismate wild-type, pH 7.5, 37°C Escherichia coli