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Literature summary for 5.4.2.9 extracted from
- Purification and characterization of phosphoenolpyruvate phosphomutase from Pseudomonas gladioli B-1 (1992), J. Bacteriol., 174, 6857-6861.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
Phosphonopyruvate | 30°C, pH 7.5 | Burkholderia gladioli |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | Km: 0.0012 mM | Burkholderia gladioli | |
| Mg2+ | Km: 0.0035 mM | Burkholderia gladioli | |
| Mn2+ | activates | Burkholderia gladioli | |
| Mn2+ | Km: 0.000016 mM | Burkholderia gladioli | |
| Zn2+ | Km: 0.0015 mM | Burkholderia gladioli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
4 * 61000, SDS-PAGE | Burkholderia gladioli |
| 220000 | - |
PAGE | Burkholderia gladioli |
| 263000 | - |
gel filtration | Burkholderia gladioli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia gladioli | - |
B-1 | - |
| Burkholderia gladioli B-1 | - |
B-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Burkholderia gladioli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Burkholderia gladioli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol, 25 mM potassium phosphate, pH 7 | Burkholderia gladioli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate | equilibrium greatly favours phosphoenolpyruvate | Burkholderia gladioli | 3-phosphonopyruvate | - |
r | |
| phosphoenolpyruvate | equilibrium greatly favours phosphoenolpyruvate | Burkholderia gladioli B-1 | 3-phosphonopyruvate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 61000, SDS-PAGE | Burkholderia gladioli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Burkholderia gladioli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | - |
27°C: about 50% of maximum activity, 45°C: about 70% of maximum activity | Burkholderia gladioli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
pH 7.5, 30 min, no loss of activity | Burkholderia gladioli |
| 30 | - |
pH 7.5, 30 min, 2% loss of activity | Burkholderia gladioli |
| 35 | - |
pH 7.5, 30 min, 51% loss of activity | Burkholderia gladioli |
| 40 | - |
pH 7.5, 30 min, 64% loss of activity | Burkholderia gladioli |
| 45 | - |
pH 7.5, 30 min, 71% loss of activity | Burkholderia gladioli |
| 50 | - |
pH 7.5, 30 min, 92% loss of activity | Burkholderia gladioli |
| 60 | - |
pH 7.5, 30 min, complete loss of activity | Burkholderia gladioli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Burkholderia gladioli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | 25°C, 30 min, highest stability in this range, pH 9.1: 50% loss of activity, pH 5.2: complete loss of activity | Burkholderia gladioli |
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