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Literature summary for 5.4.2.9 extracted from
- Purification and characterization of the Tetrahymena pyriformis P-C bond forming enzyme phosphoenolpyruvate phosphomutase (1990), Biochemistry, 29, 7059-7063.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Tetrahymena pyriformis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
Phosphonopyruvate | pH 7.5 | Tetrahymena pyriformis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | Km: 0.002 mM | Tetrahymena pyriformis | |
| Co2+ | divalent metal ion required, 50% of the activation with Mg2+ | Tetrahymena pyriformis | |
| Mg2+ | Km: 0.0058 mM | Tetrahymena pyriformis | |
| Mg2+ | divalent metal ion required: Mg2+, Co2+, Zn2+ or Mn2+, maximal activation with Mg2+ | Tetrahymena pyriformis | |
| Mn2+ | Km: 0.007 mM | Tetrahymena pyriformis | |
| Mn2+ | divalent metal ion required, 30% of the activation with Mg2+ | Tetrahymena pyriformis | |
| Zn2+ | Km: 0.007 mM | Tetrahymena pyriformis | |
| Zn2+ | divalent metal ion required, 40% of the activation with Mg2+ | Tetrahymena pyriformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Tetrahymena pyriformis |
| 81000 | - |
gel filtration | Tetrahymena pyriformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tetrahymena pyriformis | - |
ATCC 151905 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Tetrahymena pyriformis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| phosphoenolpyruvate = 3-phosphonopyruvate | mechanism | Tetrahymena pyriformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Tetrahymena pyriformis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, after quick freezing in a buffered solution, pH 7.5, 10% v/v glycerol, 50 mM triethanolamine, 0.5 mM DTT, 5 mM MgCl2, less than 20% loss of activity after 6 weeks | Tetrahymena pyriformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate | - |
Tetrahymena pyriformis | 3-phosphonopyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE | Tetrahymena pyriformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Tetrahymena pyriformis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 38 | - |
Phosphonopyruvate | - |
Tetrahymena pyriformis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Tetrahymena pyriformis |
| 7.5 | - |
assay at | Tetrahymena pyriformis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
Mg2+ | pH 7.5 | Tetrahymena pyriformis | |
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