Crystallization (Comment) | Organism |
---|---|
in complex with Mg2+ | Lactococcus lactis |
Protein Variants | Comment | Organism |
---|---|---|
D170A | 700fold reduction of kcat-value | Lactococcus lactis |
D8A | no catalytic activity | Lactococcus lactis |
D8E | no catalytic activity | Lactococcus lactis |
E169A/D170A | 1400fold reduction of kcat-value | Lactococcus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0078 | - |
beta-D-glucose 1-phosphate | mutant D170A, 25°C, pH 7.0 | Lactococcus lactis | |
0.0146 | - |
beta-D-glucose 1-phosphate | wild-type, 25°C, pH 7.0 | Lactococcus lactis | |
0.39 | - |
beta-D-glucose 1-phosphate | mutant E169A/D170A, 25°C, pH 7.0 | Lactococcus lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Km-value 0.270 mM | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | P71447 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
beta-D-Glucose 1-phosphate = beta-D-glucose 6-phosphate | a single Mg2+ coordination site accomodates water, phosphate, and the phosphorane intermediate during catalytic turnover. Bi-bi ping-pong mechanism, substrate induced-fit mechanism allows phosphomutase activity to dominate over the intrinsic phosphatase activity | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-Glucose 1-phosphate | - |
Lactococcus lactis | beta-D-Glucose 6-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
beta-D-glucose 1-phosphate | mutant E169A/D170A, 25°C, pH 7.0 | Lactococcus lactis | |
0.00384 | - |
beta-D-glucose 1-phosphate | mutant D170A, 25°C, pH 7.0 | Lactococcus lactis | |
17.1 | - |
beta-D-glucose 1-phosphate | wild-type, 25°C, pH 7.0 | Lactococcus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Lactococcus lactis |