Activating Compound | Comment | Organism | Structure |
---|---|---|---|
arsenate | increasemM s activity | Euglena gracilis | |
beta-D-Glucose 1,6-diphosphate | required | Euglena gracilis | |
beta-D-Glucose 1,6-diphosphate | Km: 0.0005 mM | Euglena gracilis |
General Stability | Organism |
---|---|
after 12 h in 0.02 M imidazole buffer, at pH 7.0, totally inactivated both at 0°C and at -20°C. Glycylglycine, N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid and phosphate at the same conditions are able to preserve most of the enzyme activity. Cacodylate, ethylmorpholidate, Tris-maleate and glycerophosphate have an intermediate action | Euglena gracilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KF | 10 mM: 75% inhibition, 17 mM: 100% inhibition | Euglena gracilis | |
phosphate | 10 mM, 20% inhibition | Euglena gracilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation, Mg2+, Co2+ or Mn2+ required, maximal activity at 0.4 mM Co2+ | Euglena gracilis | |
Mg2+ | divalent cation, Mg2+, Co2+ or Mn2+ required, maximal activity at 1-3 mM Mg2+ | Euglena gracilis | |
Mn2+ | divalent cation, Mg2+, Co2+ or Mn2+ required, maximal activity at 0.4 mM Mn2+ | Euglena gracilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
gel filtration | Euglena gracilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-Glucose 1-phosphate | Euglena gracilis | the enzyme together with a trehalose phosphorylase would constitute a new catabolic pathway for trehalose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Euglena gracilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.463 | - |
- |
Euglena gracilis |
Storage Stability | Organism |
---|---|
-70°C, 0.02 M phosphate buffer, pH 7.0, stable for at least 6 months | Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-Glucose 1-phosphate | r | Euglena gracilis | beta-D-Glucose 6-phosphate | - |
? | |
beta-D-Glucose 1-phosphate | the enzyme together with a trehalose phosphorylase would constitute a new catabolic pathway for trehalose | Euglena gracilis | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Euglena gracilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.2 | 6.0: about 35% of maximal activity, pH 8.2: about 15% of maximal activity | Euglena gracilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
0°C: about 70% loss of activity after 2 days, -20°C: about 30% loss of activity after 2 days, -70°C: no loss of activity after 2 days | Euglena gracilis |