Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of variants by site directed mutagenesis replacing Ser23 and Cys22 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.182 | - |
2,3-diphosphoglycerate | mutant S23G | Homo sapiens | |
0.281 | - |
2,3-diphosphoglycerate | mutant C22S | Homo sapiens | |
0.3 | - |
2,3-diphosphoglycerate | wild-type | Homo sapiens | |
0.583 | - |
2,3-diphosphoglycerate | mutant C22T | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-Diphosphoglycerate | Homo sapiens | in red blood cells 2,3-diphosphoglycerate is the main allosteric effector of hemoglobin, shifting the equilibrium between the oxy and deoxy conformations of hemoglobin preferentially stabilizing the unliganded form. 2,3-Diphosphoglycerate also promotes the polymerization of deoxyhemoglobin in sickle cell disease | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
-80°C, sodium phosphate buffer, 20% glycerol | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-Diphosphoglycerate | the enzyme has additional activities of EC 5.4.2.1(phosphoglycerate mutase) and EC 3.1.3.13 (bisphosphoglycerate phosphatase) | Homo sapiens | 2,3-Diphosphoglycerate | - |
? | |
1,3-Diphosphoglycerate | multifunctional enzyme for synthesis and degradation of 2,3-diphosphoglycerate | Homo sapiens | 2,3-Diphosphoglycerate | - |
? | |
2,3-Diphosphoglycerate | in red blood cells 2,3-diphosphoglycerate is the main allosteric effector of hemoglobin, shifting the equilibrium between the oxy and deoxy conformations of hemoglobin preferentially stabilizing the unliganded form. 2,3-Diphosphoglycerate also promotes the polymerization of deoxyhemoglobin in sickle cell disease | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
2,3-diphosphoglycerate | wild-type | Homo sapiens | |
0.00623 | - |
2,3-diphosphoglycerate | mutant C22S | Homo sapiens | |
0.00845 | - |
2,3-diphosphoglycerate | mutant S23G | Homo sapiens | |
0.152 | - |
2,3-diphosphoglycerate | mutant C22T | Homo sapiens |