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Literature summary for 5.3.99.3 extracted from

  • Takusagawa, F.
    Microsomal prostaglandin E synthase type 2 (mPGES2) is a glutathione-dependent heme protein, and dithiothreitol dissociates the bound heme to produce active prostaglandin E2 synthase in vitro (2013), J. Biol. Chem., 288, 10166-10175.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.99.3

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol leads to dissociation of the bound heme which is hindered by imidazole Macaca fascicularis
GSH mPGES2 has a GSH-binding motif, the GSH molecule binds at the predicted amino acid residues 148VPxL to 164DSxxI. GSH is essential for heme binding to isozyme mPGES2, structures of GSH-heme complex-binding cavity in isozyme mPGES2, overview Macaca fascicularis

Inhibitors

Inhibitors Comment Organism Structure
heme heme is bound to the enzyme subunits and alters the enzyme catalytic specificity, overview. Heme is attached to the bound GSH by an iron-sulfur bond, but heme cannot bind to mPGES2 in the absence of GSH, other sulfhydryl compounds, such as DTT and 2-mercaptoethanol, are unable to promote heme binding to isozyme mPGES2. Bound heme is oxidized by H2O2, but it does not dissociate from mPGES2 Macaca fascicularis
imidazole imidazole stacks on the bound heme and inhibits heme oxidation by H2O2, heme dissociation by DTT, and catalytic activity Macaca fascicularis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-associated prostaglandin E synthase-2 Macaca fascicularis 16020
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microsome
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 Macaca fascicularis
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(5Z,13E,15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate Macaca fascicularis reaction of heme-free isozyme mPGES2 (5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
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 ?

Organism

Organism UniProt Comment Textmining
Macaca fascicularis Q9N0A4 isozyme mPGES2
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Source Tissue

Source Tissue Comment Organism Textmining
liver
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 Macaca fascicularis
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additional information abundant tissue distribution of isozyme mPGES2 Macaca fascicularis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,13E,15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate reaction of heme-free isozyme mPGES2 Macaca fascicularis (5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
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 ?
additional information heme-free isozyme mPGES2 and heme-bound isozyme mPGES2 catalyze isomerization of prostaglandin H2 to prostaglandin E2 and conversion of prostaglandin H2 to to (12S)-hydroxy-(5Z,8E,10E)-heptadecatrienoic acid and malondialdehyde, respectively Macaca fascicularis ?
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 ?

Synonyms

Synonyms Comment Organism
membrane-associated prostaglandin E synthase-2
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 Macaca fascicularis
MPGES2
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 Macaca fascicularis
prostaglandin E2 synthase
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 Macaca fascicularis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4
-
 assay at Macaca fascicularis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Macaca fascicularis

Expression

Organism Comment Expression
Macaca fascicularis iron does not induce the enzyme expression additional information