Cloned (Comment) | Organism |
---|---|
into the vector pPICZA for overexpression in Pichia pastoris cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R126A | mutant, exhibits a glutathione-dependent reductase activity, which allows conversion of prostaglandin H2 into prostaglandin F2alpha | Homo sapiens |
R126Q | mutant, exhibits a glutathione-dependent reductase activity, which allows conversion of prostaglandin H2 into prostaglandin F2alpha | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
microsome | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
determined by SDS-PAGE and Western Blot analysis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | Homo sapiens | PGH2 | (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate | PGE2 | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O14684 | - |
- |
Purification (Comment) | Organism |
---|---|
microsomes are prepared | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | PGH2 | Homo sapiens | (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate | PGE2 | ? | |
additional information | (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate, PGH2, mutation R126A/Q creates a glutathione-dependent reductase which is able to convert prostaglandin H2 to prostaglandin F2alpha | Homo sapiens | additional information | (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate, PGF2alpha | ? |
Synonyms | Comment | Organism |
---|---|---|
microsomal prostaglandin E synthase type 1 | - |
Homo sapiens |
microsomal prostaglandin E synthase-1 | - |
Homo sapiens |
mPGES-1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
microsomal suspension is incubated on ice with PGH2, enzyme activity assay | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
enzyme activity assay | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | mutants R126A/Q possess glutathione-dependent reductase activity | Homo sapiens |