Literature summary for 5.3.99.3 extracted from

Ouellet, M.; Falgueyret, J.P.; Ear, P.H.; Pen, A.; Mancini, J.A.; Riendeau, D.; David Percival, M.
Purification and characterization of recombinant microsomal prostaglandin E synthase-1 (2002), Protein Expr. Purif., 26, 489-495.

Search for more literature for 5.3.99.3

Activating Compound

Activating Compound	Comment	Organism	Structure
captoprylsulfobetaine	10 mM, 2.6fold activation	Homo sapiens
diheptanoylphosphatidylcholine	2.2 mM, 4.8fold activation	Homo sapiens
n-dodecylmaltoside	0.3 mM, 5fold activation	Homo sapiens
n-octylglucoside	34 mM, 3.3fold activation	Homo sapiens
Triton X-100	5 mM, 4.6fold activation	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Sf9 insect cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
MK-0886	0.002 mM, 50% inhibition	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.014	-	(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate	-	Homo sapiens
0.1	-	(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate	in the presence of 0.3 mM dodecylmaltoside	Homo sapiens
0.6	-	glutathione	in the presence of 0.3 mM dodecylmaltoside	Homo sapiens
0.75	-	glutathione	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
integral to membrane	-	Homo sapiens	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17000	-	x * 17000, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O14684	-	-

Purification (Commentary)

Purification (Comment)	Organism
detergent, hydroxyapatite column	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate + reduced glutathione	i.e. prostaglandin H2	Homo sapiens	(5Z,13E)-(15S)-11alpha,15-dihydroxyprosta-5,13-dienoate + oxidized glutathione	i.e. prostaglandin E2	?

Subunits

Subunits	Comment	Organism
?	x * 17000, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
microsomal prostaglandin E synthase-1	-	Homo sapiens
mPGES-1	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
7	-	(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate	dodecylmaltoside activated	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
glutathione	-	Homo sapiens

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Release 2023.1 (January 2023)